摘要
目的探讨凝血酶敏感蛋白-1(TSP-1)和表皮生长因子受体(EGFR)在大鼠气道黏液分泌中的作用。方法将20只雄性Wistar大鼠分为对照组和内毒素组。采用HE染色和阿辛蓝-过碘酸雪夫(AB-PAS)染色进行气道上皮杯状细胞计数及检测气道黏液物质表达。采用免疫组化法检测各组大鼠黏液腺TSP-1、EGFR和MUC5AC蛋白的表达,对肺组织切片进行图像分析。结果内毒素组气道上皮杯状细胞积分、气道黏液物质、气道及肺组织TSP-1、EGFR和MUC5AC蛋白表达分别与正常对照组相比升高(P<0.05)。TSP-1、EGFR的表达与MUC5AC的表达呈正相关性(r=0.804、0.793,P<0.05)。结论大鼠气道和肺组织高表达TSP-1和EGFR,可能参与了气道黏液高分泌的的发生。
Objective To explore the expression of thrombospondin - 1 （TSP - 1 ） and epidermal growth factor receptor（EGFR） on airway mucus hypersecretion in rats. Methods Twenty male Wistar rats were divided into two groups：the endotoxin group and the control group respectively. The count of epithelial goblet cell, the expression of mucin in bronchial tissues were examined by HE staining and aleain blue - periodic and Schiff（ AB - PAS） staining. The expression of TSP - 1 ,EGFR and MUC5AC in the tung of rats were meas- ured by immunohist ochemical staining. The lung tissue sections were analyzed by image analysis. Results Comparing with the control group,the count of epithelial goblet cell, the expression of TSP - 1, EGFR and MUC5AC protein in endotoxin group were significantly higher （ P 〈 0.05 ）. The expression of TSP - 1 and EGFR were positively correlated with the expression of MUC5AC（ r = 0. 804,0.793, P 〈 0.05 ）. Conclusion The high expression of TSP - 1 and EGFR may play an important role in the pathology process of the airway mucous hypersecretion.
出处
《宁夏医学杂志》
CAS
2016年第12期1123-1125,I0001,共4页
Ningxia Medical Journal
基金
国家自然科学基金资助项目(30960142)
