百草枯诱导急性肺损伤大鼠和肽素水平变化及甘草酸二铵对其影响

The dynamic changes of copeptin in rats with acute lung injury induced by paraquat and diammonium glycyrrhizinate intervention studies

目的探讨急性肺损伤（ALI）大鼠和肽素（copeptin）水平的变化情况，进一步探究甘草酸二铵（diammonion glycyrrhizinate，DG）干预对其表达情况的影响。方法以百草枯（paraquat，PQ）建立ALl大鼠模型，将150只sD大鼠按照随机数字表法分为PQ染毒组、DG干预组和空白对照组（NS）。PQ组和DG组大鼠给予PQ100mg／kg一次性灌胃，NS组大鼠则给予等量生理盐水灌胃。DG组大鼠灌胃后立即给予DG50mg／kg腹腔注射，每24h重复给药1次。分别于12、24、48h处死动物留取标本。ELISA检测血清和肺组织中和肽素、炎症因子TNF—α和IL-10浓度，并测定肺组织湿／干质量（W／D）比值。结果在12、24、48h三个观察时段，PQ组血清和肺组织中和肽素、TNF-α和IL-10浓度均逐渐升高，肺组织W／D比值增高，差异有统计学意义（P均〈0．001）。而此三个观察时段的组间比较发现，DG干预组血清和肺组织中和肽素、TNF-α浓度降低，而IL-10浓度升高，肺组织W／D比值降低，差异有统计学意义（P均〈0．001）。结论和肽素水平可能与肺损伤的严重程度有关。DG对ALI有保护作用。

Objective To investigate the effects ofdiammonium glycyrrhizinate （DG） on the levels of copeptin,TNF-α and IL-10 in rats with acute lung injury（ALI） induced by paraquat （PQ）.Methods The PQ （100 mg/kg）were administered via a gastric tube to make an animal model of ALl in rats. Then DG （50 mg/kg per 24 h）were administered immediately by intraperitoneal injection in the DG group. The ELISA assay was applied to measuring the levels of copeptin ,TNF-α and IL-10 in lungs and blood. And the wet to dry ratio of lungs were measured. Results The levels of serum and pulmonary copeptin, TNF-α and IL-10 in PQ group were higher than those in NS group, and the pulmonary wet to dry ratio were increased （P 〈 0.001）.It demonstrated that the pulmonary edema was aggravating. While, the levels of serum and pulmonary copeptin and TNF-α in DG group were lower than those in PQ group. However, the levels of IL-10 in DG group were increased （P 〈 0.001）.And pulmonary wet to dry ratio were lower（P 〈 0.001 ）, it stated pulmonary edema were attenuated. Gonelusions Copeptin levels were probably associated with the severity of pulmonary edema. And DG had protective effect against acute lung injury.