摘要
选择5SrRNA和snRNAu6作为内参基因,比较分析其在鸡血清循环miRNA定量检测中的应用效果。结果显示,5SrRNA和snRNAu6两个内参基因在不同发育阶段血清中表达稳定,5SrRNA的Cq值在24.4~25.0之间,snRNAu6的cq值在24.0-24.5之间,组间Cq值的变化幅度小于0.6;同一测定miRNA的组内Cq值变异也较小。两种内参基因条件下,3个测定循环miRNA的表达变化规律均一致。表明5SrRNA和snRNAu6是适于鸡血清循环miRNA定量检测的内参基因。
Two control genes,SS rRNA and snRNA U6,were selected to compare the effects in chicken (G. gallus) serum circulating miRNA real-time quantitative PCR (RT-qPCR) tests. The results showed that expression levels of 5 S rRNA and snRNA U6 were relatively stable between different groups, with 24.4 to 25.0 Cq values for 5 S rRNA and 24.0 to 24.5 Cq values for snRNA U6. The within-group Cq values for each tested miRNA also had less variation. The tested three miRNAs had the same expression tendency using either 5 S rRNA or snRNA U6. The results indicated that 5 S rRNA and snRNA U6 were suitable control genes for chicken serum circulating miRNA RT-qPCR test.
作者
朱云芬
韩威
苏一军
李国辉
王洪志
张会永
殷建玫
ZHU Yunfen HAN Wei SU Yijun LI Guohui WANG Hongzhi ZHANG Huiyong YIN Jianmei(Poultry Institute,Chinese Academy of Agrieultural Seiences,Yangzhou,Jiangsu 225125)
出处
《中国家禽》
北大核心
2016年第24期18-21,共4页
China Poultry
基金
国家自然科学基金项目(31201799)
江苏省农业三新工程项目(SXGC(2015)300)
江苏省农业科技创新项目(CX(15)1009)