东亚特有珍稀蕨类植物岩穴蕨(碗蕨科)高通量转录组测序及分析

De novo transcriptome analysis of the rare fern Monachosorum maximowiczii(Dennstaedtiaceae) endemic to East Asia

岩穴蕨(Monachosorum maximowiczii)隶属于碗蕨科稀子蕨属,是东亚中高海拔地区所特有的珍稀濒危植物。为了在分子水平对该物种有进一步的认识,本文首次利用二代高通量测序技术(RNA-seq)对岩穴蕨进行了转录组测序分析。通过Illumina Hiseq 2500测序平台,共计获得4.95 Gb原始数据(raw data),经过滤后得到4.83 Gb有效数据(clean reads),并进行从头组装得到了101,448条unigene。其中,54,106条unigene预测到完整的开放阅读框。我们利用目前已知的51个植物基因组数据,对岩穴蕨的unigene进行了详尽的功能注释,并通过GO、COG、KEGG注释进一步了解了这些编码基因的作用方式、特征以及所参与的代谢通路。同时,转录因子分析结果也为岩穴蕨的环境适应机制研究提供了初步线索。

Monachosorum maximowiczii, which belongs to the family Dennstaedtiaceae, is a unique fern rarely distributed in the middle-high altitudinal areas in East Asia. In order to identify the molecular basis of this species at the transcriptome level, a second generation sequencing technology （RNA-seq） was employed here for the first time. Using Illumina Hiseq 2500 platform, a total of 4.95 Gb raw data were obtained. After filtration, 4.83 Gb clean reads were kept and 101,448 unigene were generated using de novo assembly. Among them, 54,106 unigene were predicted to have a complete open reading frame. The functional annota- tion of the nnigene obtained here was carried out using the Phytozome vl 1.0 databases. The gene function pattem and participated pathway were further explored based on GO, COG and KEGG annotation. Meanwhile, transcription factors prediction and analysis will provide preliminary insights into mechanisms of Monacho- sorum maximowiczii for environmental adaptation.