刺五加注射液预处理对大鼠肾缺血再灌注损伤的影响

Effect of Acanthopanax senticosus Pretreatment on Acute Renal Ischemic Reperfusion Injury in Rats

该研究探讨了刺五加(Acanthopanax senticosus,AS)注射液预处理对大鼠肾缺血再灌注损伤(ischemic reperfusion injury,IRI)的保护作用及其可能机制。采取单动脉夹闭法建立大鼠肾缺血再灌注损伤模型。检测各组大鼠血清肌酐(Scr)、尿素氮(blood urea nitrogen,BUN)、丙二醛(malondialdehyde,MDA)、血液中白介素-6(interleukin-6,IL-6)、尿液肾损伤分子-1(kidney injury molecule-1,KIM-1)、6-乙酰-B-D-氨基葡萄糖苷酶(6-acetyl-B-D-glucosaminidase,NAG)的含量。苏木精–伊红染色(hematoxylin-eosin staining,HE)光镜下观察各组大鼠肾组织的病理变化。免疫组化法测定各组大鼠肾组织中细胞间黏附分子-1(intercellular adhesion molecule-1,ICAM-1)蛋白质水平。结果表明,HE染色光镜下观察,IRI组及AS组肾组织出现病理改变,对照组无明显病理变化,而AS组大鼠肾组织损伤较IRI组减轻。AS预处理大鼠肾IRI,可明显降低血清Scr、BUN、MDA、IL-6、尿液KIM-1、NAG量及肾组织中ICAM-1蛋白的表达。认为AS预处理对大鼠急性肾IRI具有明显保护作用,可能与其可以减轻大鼠IRI的炎性损伤反应有关。

This work aimed to investigate the protective effect ofAcanthopanax senticosus （AS） injection on rats with acute renal ischemic reperfusion injury （IRI） and its possible mechanism. In this study, rats with acute renal ischemia reperfusion injury model was constructed by single-artery occlusion. Puncture bladder for urinary to measure kidney injury molecule-1 （KIM-1）, 6-acetyl-B-D-glucosaminidase （NAG） by enzyme-linked immunosorbent-assay （ELISA）. The levels of serum Scr, BUN, IL-6, MDA were measured, respectively. Pathological changes of renal tissues were observed by hematoxylin-eosin staining （HE） staining technique. The protein levels of intercellular adhesion molecule-1 （ICAM-1） in renal tissues were detected by immunohistochemical method. The results of HE staining showed that IRI group and AS group had a certain degree of pathological changes, while all these pathological changes relieved in AS group but found no significant changes in control group. In AS group, levels of serum Scr, BUN, MDA, IL-6, KIM- 1 and NAG content of urinary, the protein levels of ICAM- 1 in renal tissues were significantly lower compared with IRI group. After acute renal ischemic reperfusion injury in rats, AS pretreatment could reduced levels of serum Scr, BUN, MDA, IL-6, KIM-1 and NAG content of urinary, the protein levels of ICAM-1 in renal tissue. AS could protect the kidney against renal ischemia reperfusion injury. That might be realized by its inhibition of inflammation.