依折麦布干预对2型糖尿病小鼠体内脂肪因子和胰岛素抵抗的影响

Effects of Ezetimibe on Fat Factors and Insulin Resistance in Db/db Mice

目的依折麦布干预对2型糖尿病模型db/db小鼠体内脂肪因子和胰岛素抵抗(insulin resistance,IR)的影响。方法将40只8周龄雄性db/db小鼠随机均分为依折麦布组和db/db对照组,分别给予依折麦布10 mg/(kg·d)和安慰剂灌胃。另选择20只同周龄非糖尿病(db/m小鼠)作为对照组(db/m组)。3组小鼠分别于治疗前和治疗后6周测定低密度脂蛋白(lowdensity lipoprotein,LDL)、甘油三酯(triglyceride,TC)、空腹血糖(fasting blood glucose,FBG)、空腹胰岛素(fasting serum insulin,FSI),并计算胰岛素敏感指数(insulin sensitivity index,ISI),脂联素(adiponectin,APN),趋化素水平。结果给药6周后,db/db组血清趋化素明显升高,APN水平明显下降;而依折麦布组小鼠趋化素明显下降,APN水平明显升高,治疗前后比较差异均具有统计学意义(P<0.05)。给药6周后趋化素、APN水平组间比较差异均具有统计学意义(P<0.01)。依折麦布与△趋化素呈负相关,与△APN呈正相关;△趋化素与△FBG、△FSI、△ISI呈正相关;△APN与△FBG、△FSI、△ISI呈负相关。结论依折麦布可以明显调节db/db小鼠体内趋化素和APN水平,促进胰岛素分泌,改善胰岛素抵抗(insulin resistance,IR)程度。

Objective To evaluate the effects of Ezetimibe on fat factors and insulin resistance（IR）in db/db mice.Methods Forty male 8-week-old mice were divided randomly and equally into Ezetimibe group and db / db group,and received gavage in Ezetimibe and placebo,respectively.Another twenty mice with peer group were selected as control group（db / m group）.Low density lipoprotein（LDL）,triglyceride（TC）,fasting blood glucose（FBG）and fasting serum insulin（FSI）of mice was measured in three group 6 months before and after treatment,meanwhile,insulin sensitivity index（ISI）,chemerin and adiponectin（APN）were calculated.Results After 6 weeks treatment,the levels of serum chemerin in db / db group were significantly increased while APN were decreased（P〈0.05）.The levels of chemerin in Ezetimibe group were obviously reduced and APN were upgraded（P〈0.05）.The differences of chemerin and APN leves were statistically significant in comparison among groups after 6 weeks（P〈0.01）.Ezetimibe was negatively related with △Chemerin but positively associated with △APN.△Chemerin was positively related with △FBG,△FSI and △ISI.△APN was negatively associated with △FBG,△FSI and△ISI.Conclusion Ezetimibe can improve insulin resistance and insulin secretion by regulating chemerin and APN in db/db mice.