水溶性荧光碳点的制备及其在大肠杆菌中成像

The Preparation of Water-soluble Fluorescent Carbon Dots and Its Imaging in Escherichia coli

荧光碳点是一种以碳元素为主体的新型荧光纳米材料，也是目前研究最热门的碳纳米材料之一。而水溶性荧光碳点的合成也引起了研究者的广泛关注。本研究以小米为原料，采用水热法一步合成水溶性蓝色荧光碳点，通过实验证明此种碳点粒径均一，约为2nm。并在pH为5～10下荧光强度比较稳定。合成的碳点已经成功的应用到大肠杆菌中成像，标志着这种碳点可以作为高性能的荧光探针。

Fluorescent carbon dot is a kind of carbon element as the main body of new fluorescent nanomaterials, and it is also one of the most popular carbon nano materials. The synthesis of water-soluble fluorescent carbon dots caused the extensive attention of the researchers. In this study, we used the method of hydrothermal method to synthesize water soluble blue fluorescent carbon dots by hydrothermal method. The results showed that the particle size of this kind of carbon dots was uniform, which was about 2 nm. And fluorescence intensity of pH was relatively under 5-10. Synthetic carbon dots have been successfully applied to the imaging of E. coli, marking this carbon point can be used as high performance fluorescent probes.