微生物生产L-苏氨酸的代谢工程研究进展

Advances in Microbial Metabolic Engineering to Increase L-Threonine Production

L-苏氨酸作为一种必需氨基酸被广泛用于食品、饲料、医药及化妆品行业。目前L-苏氨酸主要通过微生物发酵法生产。代谢工程技术的应用为菌种选育开辟了有效途径,使在现有高产基础上进一步提高氨基酸的产量成为可能。作者对两大氨基酸生产菌——大肠杆菌和谷氨酸棒杆菌中的L-苏氨酸生物合成相关途径、代谢调控机理以及运用代谢工程技术提高L-苏氨酸产量所取得的成果进行了系统综述。

As an essential amino acid for mammals, L-threonine has a wide application in the food, feeds,pharmaceutical and cosmetics industries. To date, L-threonine is almost exclusively produced through microbial fermentation. Metabolic engineering provides an effective means to strain development and thus to enhancing the L-threonine production. In this article,the pathway and regulation of L-threonine in the major industrial strains,Corynebacterium glutamicum and Escherichia coli are summarized,and advances on metabolic engineering to increase L-threonine production are reviewed.