富硒螺旋藻光谱特性及其含硒蛋白质组分析

Infrared Spectrum,UV-visible Absorption Spectral Characteristics and Proteomic Analysis of Selenium Enriched Spirulina Platensis

以富硒螺旋藻（Selenium enriched spirulina platensis,Se-SP）为材料,采用红外、紫外-可见吸收光谱分析Se-SP的光谱特征,并利用二维电泳技术（Two-dimensional electrophoresis,2DE）分离、飞行时间质谱（Matrix assisted laser desorption ionization time of flight,MALDI-TOF）鉴定和电感耦合等离子体质谱（Inductively coupled plasma mass spectrometry,ICP-MS）检测硒含量,对Se-SP蛋白质组及其含硒蛋白质分布进行初步鉴定和分析。结果显示,硒对螺旋藻蛋白质组中多种蛋白质表达有一定影响,观察到对捕光色素蛋白如藻蓝蛋白（Phycocyanin,PC）、别藻蓝蛋白（Allophycocyanin,APC）和Robisco大亚基及超氧化物歧化酶（Superoxide Dismutase,SOD）等蛋白质具有明显上调作用。硒在Se-SP蛋白质组中存在较为普遍,但分布相对集中在pH 4~7、分子质量14.4~43 kDa的蛋白质中。硒在藻体特定蛋白质中的存在形式还有待深入研究和鉴定。

Infrared spectrum, UV-visible absorption spectrum and differential expression of proteome between Spirulina platensis （ SP ） and selenium enriched SP （ Se-SP ） were investigated. The selenium containing proteins were separated from the total proteins in Se-SP by a proteomic approach of two-dimensional electrophoresis （ 2 DE ） , identified by matrix assisted laser desorption ionization time-of-flight mass spectrometry （ MALDI-TOF-MS ） , and determined by inductively coupled plasma mass spectrometry （ ICP-MS） . Results showed that Se enrichment cultivation could significantly up-regulate the expression of pigment proteins such as α/β subunits of phycocyanin （ PC ） and allophycocyanin （ APC ） which involved in light harvest. The large subunits of Rbisco and superoxide dismutase were also increased obviously in Se-SP. In addition, it was found that Se was abundantly distributed in the range of pH 4-7 and MW of 14. 4-43 kDa in the 2DE gel. Individuals of Se containing protein should be explored in Se-SP in further studies.