3D石墨烯修饰的猛杀威分子印迹电化学传感器的制备

Preparation of Promecarb Electrochemical Sensor Based on 3D-Graphene and Molecularly Imprinted Polymer

以猛杀威为模板分子、丙烯酰胺（AM）为功能单体、马来松香丙烯酸乙二醇酯（EGMRA）为交联剂、自制的3D石墨烯（3D-rGO）为增敏材料,在玻碳电极表面合成分子印迹聚合物,制备检测猛杀威的分子印迹电化学传感器。运用扫描电镜（SEM）对自制3D石墨烯的形貌进行了表征,通过循环伏安法（CV）、差分脉冲伏安法（DPV）和交流阻抗法（EIS）对猛杀威传感器的性能进行了研究。结果表明,猛杀威的浓度在8.0×10^-8~8.0×10^-6mol/L范围内与响应电流值呈良好的线性关系,线性相关系数R=0.9954,检出限为7.3×10^-8mol/L（S/N=3）,猛杀威分子印迹敏感膜的印迹因子β=3.88,且相对于3种结构类似物的选择因子α〉〉1,说明此传感器具有良好的选择性。将此传感器应用于生菜样品检测,加标回收率在96.7%~98.7%之间。

A sensitive electrochemical sensor for promecarb was prepared based on molecularly imprinted strategy by thermal polymerization. The molecularly imprinted polymers （ MIP ） were synthesized by using promecarb as template molecule, acrylamide （ AM） as functional monomer, ethylene glycol acrylate, maleic rosin ester （EGMRA） as a cross-linking, homemaking 3D graphene as sensitizing materials to modify glassy carbon electrode surface, and synthesize the promecarb molecular imprinting electrochemical sensor. 3D graphene was characterized by scanning electron microscopy （ SEM） . The properties of the sensor were tested via cyclic voltammetry （ CV ） , differential pulse voltammetry （ DPV ） and electrochemical impedance spectroscopy （ EIS ） . The results showed that promecarb concentration in the range of 8.0 ×10^-8 -8. 0×10^-6 mol/L had a good linear relationship （linear correlation coefficient R=0. 9954） with current, and the detection limit was 7 . 3×10^-8 mol/L （ S/N=3 ） . The imprinted factorβof promecarb molecularly imprinted sensitive film was 3. 88, the selection factorαwas far greater than 1 compared to the three structural analogs. The imprinted sensor was successfully used to determine promecarb in lettuce samples with recoveries ranging from 96. 7% to 98. 7% by using standard addition method.