基于金属有机框架结构的分子印迹聚合物电化学传感器检测猪尿中苯乙醇胺A

Molecularly Imprinting Polymer Electrochemical Sensor Based on Structure of Metal Organic Frameworks for Determination of Phenylethanolamine A in Swine Urine

以苯乙醇胺A(PEA)为模板分子、对氨基苯硫酚(PATP)为功能单体和有机配体、金纳米颗粒(AuNPs)为金属中心,通过原位电聚合在金电极表面成功制备了具有金属有机框架(MOFs)结构的分子印迹聚合物(MIP)复合膜,构建了具有分子识别特性的复合膜电化学传感器,实现了对PEA的灵敏检测。采用电化学技术和扫描电镜对修饰电极进行表征。优化了扫描速率、pH值、洗脱时间和富集时间等检测条件。在优化条件下,此电化学传感器对PEA在1.0×10^(13)~1.0×10~9mol/L范围内有很好的线性关系,相关系数达到0.996,检出限为1.0×10^(13)mol/L。在猪尿液中添加不同浓度的PEA,回收率在92.7%~108.4%,相对标准偏差小于8.0%。本方法对实际猪尿中PEA的检测具有很好的灵敏度、特异性和稳定性。

A supersensitive molecular imprinting polymer （ MIP ） electrochemical sensor based on the structure of metal organic frameworks （ MOFs） for detection of phenylethanolamine A （ PEA） in swine urine samples was developed. In this sensor, PEA was used as template, p-aminothiophenol was selected as functional monomer and organic ligand, and Au nanoparticles were used as metal center to fabricate a composite membrane with molecular recognition property on gold electrode by in situ electrochemical polymerization. The characterizations of modified electrode were performed with electrochemical platform and scanning electronic microscope. The analytical conditions including scan rate, pH, elution time and incubation time and so on were optimized. Under the optimum conditions, the limit of the developed MOFs-MIP electrochemical sensor for the detection for PEA was as low as 1 . 0×10^-13 mol/L and the linear rang was from 1 . 0×10^-13 to 1 . 0×10^-9 mol/L with correlation coefficient of 0 . 996 . The recoveries of PEA were from 92 . 7% to 108 . 4% under different spiked levels of PEA in animal urine and the relative standard deviation （RSD） was below 8. 0%. The developed method possessed many advantages such as high sensitivity, specificity and stability.