黎药枫蓼肠胃康颗粒对幼龄大/小鼠的抗炎、镇痛、保护胃黏膜及解痉作用研究

Study on Anti-inflammatory,Analgesic,Gastric Mucosa-protective and Spasmolysis Effects of Li Medicine Fengliao Changweikang Granule

目的:考察黎药枫蓼肠胃康颗粒(简称FMCWK)对幼龄大/小鼠的抗炎、镇痛、保护胃黏膜及解痉作用。方法:将60只SD幼龄大鼠随机分为空白组(蒸馏水)、保和丸组(阳性对照中药,以生药计给药剂量为0.98 g/kg)、奥美拉唑组(阳性对照化学药,4.36×10^(-3)g/kg)和FMCWK低、中、高剂量组(以生药计给药剂量分别为4.88、9.75、19.50 g/kg),每组10只;ig给药,每天1次,连续给药7 d后测定大鼠胃液的分泌量、p H值、总酸度、1 h总酸排出量及胃蛋白酶活力。将70只SD幼龄大鼠除按上述分组外,另设胃黏膜损伤模型组(蒸馏水);每天ig给药1次,连续给药4 d后测定大鼠胃黏膜损伤指数。分别取60只KM幼龄小鼠随机分为空白组(蒸馏水)、吲哚美辛组(阳性对照,9.79×10-3g/kg)和FMCWK低、中、高剂量组(以生药计给药剂量分别为9.74、19.48、38.96g/kg),每组12只;每天ig给药1次,连续给药4 d后分别采用腹腔毛细管通透性实验检测小鼠腹腔中伊文思蓝含量和乙酸致痛法测定小鼠15 min内的扭体次数,以考察FMCWK的抗炎、镇痛作用。取2 cm幼龄大鼠胃条,Ba Cl2致痉挛,考察终质量浓度为29、87、294 g/L(以生药计)的FMCWK和22 g/L(以生药计)的保和丸对胃条张力的影响并计算解痉百分率,考察其解痉作用。结果:与空白组比较,高剂量FMCWK可明显升高大鼠胃液p H值、降低胃液的总酸度和1 h总酸排出量、减少小鼠腹腔内伊文思蓝含量和15 min内扭体次数(P<0.05或P<0.01);各质量浓度FMCWK均能明显降低大鼠胃条张力、提高胃条解痉百分率(P<0.01)。与模型组比较,中、高剂量FMCWK均可明显降低大鼠胃黏膜损伤指数(P<0.05或P<0.01)。结论:FMCWK对幼龄大/小鼠具有一定的抗炎、镇痛、保护胃黏膜及解痉作用。

OBJECTIVE：To investigate anti-inflammatory,analgesic,gastric mucosa-protective and spasmolysis effects of Li medicine Fengliao changweikang granule（FMCWK）in juvenile rats/mice. METHODS：60 SD juvenile rats were randomly divided into blank group（distilled water）,Baohe pills group（TCM positive control,0.98 g/kg,calculated by crude drug）,omeprazole group（chemicals positive control,4.36 × 10^-3g/kg） and FMCWK low- dose,medium- dose and high- dose groups（4.88,9.75,19.50 g/kg,calculated by crude drug）,with 10 rats in each group. They were given relevant medicine intragastrically,once a day,for consecutive 7 d. The secretion of gastric juice,p H value,total acidity,1 h total acid output and the activity of pepsase were all determined. 70 SD juvenile rats were grouped with same method,and gastric mucosal lesion model group（distilled water）was also set up. They were given relevant medicine intragastrically,once a day,for consecutive 4 d. The index of gastric mucosal lesion was determined. 60 KM juvenile mice were randomly divided into blank group（distilled water）,indometacin group（positive control,9.79 × 10^-3g/kg） and FMWK low- dose,medium- dose and high- dose groups（4.88,9.75,19.50 g/kg,calculated by crude drug）,with 12 mice in each group. They were given relevant medicine intragastrically,once a day,for consecutive 4 d. The peritoneal capillary permeability test was adopted to determine the content of Evans blue solution in mice;acetic acid induced pain method was used to determine the number of writhing within 15 min;anti-inflammatory and analgesic effects of FMCWK were investigated. The gastric fundus strips with the length of 2 cm was collected to induce spasm by Ba Cl2. The effects of FMCWK（29,87,294 g/L,calculated by crude drug）and Baohe pills（22 g/L,calculated by crude drug）on gastric fundus strips tension were investigated and the spasmolysis percentage was also calculated to investigate its spasmolysis effect. RESULTS：Compared with blank group,high-dose FMCWK significantly increased p H of gastric juice,and decreased total acidity,1 h total acid output of rats,decreased the content of Evans blue and the number of writhing within 15 min in mice（P〈0.05 or P〈0.01）;different concentrations of FMCWK significantly decreased gastric fundus strips tension while decreased spasmolysis percentage of rats（P〈0.01）.Compared with model group,medium-dose and high- dose FMCWK significantly decreased the index of gastric mucosal lesion in rats（P〈0.05 or P〈0.01）. CONCLUSIONS：FMCWK possesses certain anti- inflammatory,analgesic,gastric mucosa- protective and spasmolysis effects for juvenile rats/mice.