miR197-p53途径在三阴性和激素受体依赖型乳腺癌中的研究

Study of miR197-p53 pathway in triple negative and hormone receptor dependent breast cancer

目的:探究miR197在三阴性和激素受体依赖型乳腺癌中的表达、作用机制以及预后。方法:收集健康女性(乳腺癌疑似患者活检后正常)乳腺样本(n=10),三阴性乳腺癌患者(n=40),激素受体依赖型[雌激素和(或)孕激受体阳性、人表皮生长因子受体-2(Her-2)阴性]乳腺癌患者(n=40)的肿瘤样本。通过荧光实时定量PCR检测三组样本的miR197表达。通过蛋白免疫印迹法(Western Blotting),检测正常女性和不同类型乳腺癌患者p53以及凋亡通路蛋白。结果:低表达miR197占三阴性乳腺癌患者的77.5%(31/40)、占激素受体依赖型乳腺癌的42.5%(17/40)的和正常女性的20.0%(2/10),分别P<0.05、P<0.005。miR197表达高的细胞p53的表达较低,低表达miR197细胞Caspase-3、Caspase-9表达增高,凋亡显著。结论:miR197-p53途径抑制细胞凋亡,与乳腺癌不良预后相关。

Objective： To investigate the expression and prognosis mechanism of miR197 in invasive breast cancer. Methods： Collecting serum of healthy women（ n = 10）,patients with hormone receptor dependent（ ER or / and PR positive,Her- 2 negative） breast cancer（ n = 40）,triple patients with negative breast cancer（ n = 40） tumor samples.Detecting the miR197 in serum samples of three groups through the Real- time PCR. Detecting the expression of apoptosis protein in two groups of patients with breast cancer by Western Blotting. Results： The low expression of miR197 in patients with 42. 5 % patients with triple negative breast cancer（ 31 /40）. Hormone receptor dependent breast cancer（ 17 /40） and 20. 0 % normal women（ 2 /10）,P 0. 05. miR197 expression in breast cancer patients with low expression of p53 was higher,and it was more likely to occur apoptosis. Conclusion： The expression of miR197 in breast cancer cells is low,and it is associated with poor prognosis.