黄芩甙锌对冰醋酸致实验性大鼠胃溃疡的影响

Effects of Baicalin-Zinc on gastric ulcer induced by glacial acetic acid in rats

目的:观察黄芩甙锌(HBZn)对冰醋酸致大鼠胃溃疡保护作用。方法:采用Okzbe冰醋酸法改进后建实验性胃溃疡模型,随机分为8组:假手术组、模型组、黄芩甙(HB)组(0.35mg/kg)、葡萄糖酸锌组(0.07mg/kg)、奥美拉唑组(4.00mg/kg)、HBZn低、中、高剂量组(0.013、0.025、0.05mg/kg)。体式显微镜下计算溃疡面积;比色法测定血浆丙二醛(MDA)、一氧化氮(NO)含量;透射电镜显微技术电镜观察给药后各组胃黏膜组织形态学变化。结果:与模型组比较,HBZn低、中、高剂量组可减少溃疡面积(P<0.05),降低血浆中MDA水平,升高NO水平(P<0.05),HBZn高剂量组与奥美拉唑作用相当,且明显优于HB组和葡萄糖酸锌组(P<0.05)。同时HBZn可改善胃黏膜坏死、炎细胞浸润、组织水肿及线粒体损伤。HB组和葡萄糖酸锌组作用不如HBZn低、中、高剂量组。结论:HBZn对冰醋酸所致大鼠胃黏膜溃疡具有保护作用,该作用可能与抑制炎性反应、拮抗氧化应激、保护线粒体结构功能有关。

Objective：To study the protective effect of Baicalin-Zinc（HBZn） on gastric ulcer induced by glacial acetic acid in rats.Methods：The rat model of experimental gastric ulcerwas modified by Okzbe glacial acetic acid method.SD rats were randomly divided into 8 groups：sham operated group,model group,Baicalin（HB） group with equimolar concentration（0.35mg/kg）,Zinc gluconate with equimolar concentration of Zn group（0.07mg/kg）,Omeprazole group（4.00mg/kg）,HBZn low,medium,high dose group（0.013,0.025,0.05mg/kg）.Ulcer area was calculated under stereo microscope;the contents of malondialdehyde（MDA）,nitric oxide（NO） in plasma were determined by colorimetry;morphological changes of gastric mucosa were observed by transmission electron microscopy.Results：Compared with the model group,the HBZn low,medium,high dose group could reduce the area of ulcer（P〈0.05）,decrease the level of MDA in plasma and increase the level of NO（P0.05）,HBZn high dose group had equal effect with the positive Omeprazole group.And it was significantly better than HB group and Zinc gluconate group（P0.05）.At the same time,HBZn could improve gastric mucosal necrosis,inflammatory cell infiltration,tissue edema and mitochondrial damage.The HB group and Zinc gluconate group were less effective than HBZn low,medium,high does group.Conclusion：The protective effect of HBZn on gastric mucosal ulceration in rats induced by glacial acetic acid may be related to inhibiting inflammatory reaction,antagonizing oxidative stress and protecting mitochondrial structure and function.