基于CdSe/ZnS量子点荧光猝灭法测定硫胺素

Fluorescence Quenching Determination of Thiamin Based on CdSe/ZnS Quantum Dots

以CdSe/ZnS量子点为荧光探针,基于硫胺素(VB1)与CdSe/ZnS量子点间通过静电作用而有效猝灭CdSe/ZnS量子点荧光强度的机制,建立了一种可快速测定VB_1的荧光检测方法。在最优实验条件下(pH 7.4,反应时间25 min),硫胺素(VB_1)浓度在0.01~1μmol/L时,CdSe/ZnS量子点荧光猝灭变化强度与硫胺素(VB_1)浓度呈良好的线性关系:F_0/F=0.67c_(VB1)+1.05(R=0.999 2),方法检出限为5.1×10^(-3)μmol/L,相对标准偏差为1.09%。该方法可用于人体尿样中VB_1的快速测定。

A new fluorescent probe analysis method for determination of thiamine（VB1） was developed based on fluorescent quenching function of thiamine（VB1） for CdSe/ZnS quantum dots.The fluorescence quenching was caused by the electrostatic inter-action from thiamine（VB1） to CdSe/ZnS QDs.Under the optimal conditions（p H 7.4,reaction time of 25 min）,when the concentration of thiamine（VB1） is in the range of 0.01~1 μmol/L,the fluorescence quenching change strength of Cd Se/Zn S quantum dots has a good linear relationship with concentration of thiamine（VB1）：F0/F=0.67c（VB1）＋ 1.05（R=0.999 2）.The detection limit and relative standard deviation were 5.1×10^-3μmol/L and 1.09%,respectively.Finally,this method was successfully used to detect thiamine（VB1） in human urine.