应用亚临界水技术对枇杷叶中熊果酸的提取工艺优化研究

Optimization research of extraction process of ursolic acid from loquat leaf with subcritical water technology

因实用的药用价值,熊果酸的亚临界水萃取技术受到相关研究者的关注。为有效提高熊果酸的提取率,以亚临界水萃取过程中的萃取时间、萃取温度、溶剂流速和系统压力为可变因素,通过设计正交试验及应用高效液相色谱对熊果酸进行定量测定,最终确定最佳的提取工艺:原料的粒径60目、提取温度200℃、提取时间75min、系统压力15 MPa,提取率达到70.09%。亚临界水萃取方法不仅提高熊果酸的提取率,还是一种新型的绿色萃取技术,具有良好的发展前景。

The subcritical water extraction technology of bear acid is concerned by the researchers because of its practical medicinal value.In order to improve the extraction rate of the ursolic acid,this paper takes extraction time,extraction temperature,solvent flow rate and system pressure in the process of subcritical water extraction as variable factors,by designing orthogonal test and using high performance liquid chromatography to quantitative analysis ursolic acid,and finally determines the best extraction process：particle size 60 mesh of raw material,extraction temperature of 200℃,extraction time of 75 min,system pressure of 15 MPa,and extraction rate reached 70.09%.The subcritical water extraction method not only improves the extraction rate of the ursolic acid,but also serves as a new green extraction technology with development prospects.