P-AKT、FASN蛋白与骨肉瘤的相关性研究

The study of relationship between P-AKT and FASN in osteosarcoma

目的探讨骨肉瘤组织中P-AKT及FASN中的表达情况及其与预后的关系。方法采用免疫组织化学方法及Western blot法检测P-AKT、FASN在50例骨巨细胞瘤组织及50例骨肉瘤组织中的表达,并分别分析其与预后的关系。结果免疫组织化学结果显示,P-AKT在骨巨细胞瘤中的阳性表达率为62.00%,显著低于其在骨肉瘤中的表达80%。FASN在骨巨细胞瘤中的阳性表达率为26%,显著低于其在骨肉瘤中的表达62%(P<0.05)。Western blot检测结果与免疫组织化学结果一致。直线相关分析显示,P-AKT的表达与软组织浸润及转移密切相关,FASN的表达与软组织浸润相关;直线相关显示,P-AKT及FASN的表达呈显著正相关。结论P-AKT的表达与软组织浸润及转移密切相关,FASN的表达与软组织浸润相关,且均在骨肉瘤中表达上调,提示两种基因可能与骨肉瘤的发生发展有关。

Objective This work aimed to investigate the relationship between P-AKT and FASN in osteosarcoma. Methods SP immunohistochemical and Western blot analysis were used to detect the expression of P-AKT and FASN in osteosarcoma （50 cases）and giant cell tumor of bone （50 cases）, and their relationships to clinical and pathological factors were analyzed. Results IHC showed that P-AKT was expressed in 62.00% （31/50） of giant cell tumors,which was lower than that in osteosarcoma （80%）（40/ 50） （P 〈 0.05）. FASN was expressed in 26% （13/50） of giant cell tumors, which was lower than that in osteosarcoma （62%）（31/50） （P 〈 0.05）, the same results were observed in Western blot. The expression P-AKT was correlated with soft tissue invasion and metastasis, and FASN was correlated with metastasis. There was a positive relationship between the expression of P-AKT and FASN in the same sample. Conclusion The expression of P-AKT is correlated with soft tissue invasion and metastasis, and FASN correlated with metastasis and is highly expressed in osteosarcoma, which suggests that P-AKT and FASN may promote the genesis and development of osteosarcoma.