二藤通痹颗粒抗类风湿关节炎活性和机制研究

Anti-rheumatoid Arthritis Activity and Mechanism of Erteng Tongbi Granule

目的:探讨医院制剂二藤通痹颗粒(ETTB)抗类风湿关节炎疗效和作用机制。方法:以DBA/1小鼠胶原诱导性关节炎(CIA)为动物模型,分别给予高、中、低三个剂量的ETTB,并设空白对照组、模型对照组和甲氨蝶呤阳性组。观察ETTB对CIA小鼠的体质指数、关节炎指数、踝关节破坏改善以及血清细胞因子肿瘤坏死因子-α(TNF-α)和白细胞介素-6(IL-6)表达的影响。结果:与空白对照组比较,ETTB高剂量组(6 g·kg^(-1))可显著降低CIA小鼠的关节炎指数(P<0.05),给药第14天关节破坏程度明显改善。ELISA结果显示,ETTB高剂量组较模型对照组可显著抑制细胞因子TNF-α和IL-6分泌(P<0.01)。结论:医院制剂二藤通痹颗粒具有抗类风湿关节炎作用,其抗炎活性可能与抑制细胞因子分泌有关。

Objective： To discuss the anti-rheumatoid arthritis activity and mechanism of hospital preparation Erteng Tongbi gran- ule （ETTB）. Methods： The collagen-induced arthritis （CIA） animal model in DBA/1 mice was established and treated with high, medium and low dosage of ETTB. Blank control, model control and methotrexate group were also established. The influences of ETI＇B on BMI, arthritis index, ankle joint destruction and expression of serum cytokine TNF-α and IL-6 in CIA mice were observed. Re- suits： Compared with the control group, ETTB at high dose （6g kg-1 ） could significantly decrease the arthritis index of CIA mice （P 〈 0.05）, and the joint damage on the 14th day was also significantly improved. ELISA results showed that ETTB at high dose could significantly inhibit the secretion of cytokine TNF-α and IL-6 when compared with the model group （ P 〈 0.01）. Conclusion： Hospital preparation ETrB has anti-rheumatoid arthritis activity, and its mechanism may be related with the inhibition of cytokine secretion.