灯盏乙素纳米混悬液的制备及其表征

Preparation and Characterization of Scutellarin Nanosuspension

目的:制备灯盏乙素纳米混悬液(SCU-NS),并考察制备过程中的主要影响因素。方法:通过试验确定制备工艺参数,采用单因素试验初选SCU-NS的制备条件,以Zeta电位为评价指标,采用正交试验设计法确定最优处方。结果:所得较优的处方为:0.5 g药物、0.1 g泊洛沙姆188、0.2 g卵磷脂、0.05 g十二烷基硫酸钠(SDS)、0.05 g羟丙甲纤维素E5(HPMC E5),制得的SCU-NS粒径为(122±4)nm,Zeta电位为(-25.5±0.6)m V。透射电镜结果显示SCU-NS呈球形且粒径均一,30 min时的溶出率大于90%。结论:纳米混悬液能显著提高药物的溶出度。

Objective： To prepare scutellarin nanosuspension （SCU-NS） and study the main influencing factors in the prepara- tion. Methods： The technology parameters were determined, and then the influencing factors of SCU-NS were studied. The optimal formula was confirmed by orthogonal design with zeta potential as the evaluation index. Results： The optimal formula process was as follows ： drug amount was 0.5 g, Pluronic F68 amount was 0.1 g, phospholipid amount was 0.2 g, SDS amount was 0.05 g and HPMC E5 amount was 0.05 g. The average particle size and the zeta potential of SCU-NS was （ 122 ± 4） nm and （ - 25.5 ± 0.6） mV, respectively. The result of transmission electron microscope showed that SCU-NS was spherical and uniform, and the dissolution of SCU-NS in 30 min was more than 90%. Conclusion： Nanosuspension can significantly enhance the dissolution of SCU.