Box-Behnken效应面法优化板蓝根药材闪式提取工艺

Optimization of Flash Extraction for Isatidis Radix by Box-Behnken Experimental Design

目的:采用Box-Behnken效应面法,优选板蓝根药材最佳闪式提取工艺。方法:采用闪式提取方法,以提取电压、料液比、提取时间为自变量,采用Hassan方法计算干浸膏得率、表告依春提取率的总评"归一值(OD)",建立总评归一值与自变量之间的数学关系,用效应面法预测最佳工艺条件。结果:最佳提取工艺为:提取电压88 V、料液比19.62、提取时间2.03 min;干浸膏率及表告依春提取率分别为37.902%、0.1887%。结论:模型预测值与实验观察值接近,表明采用Box-Behnken响应面法优化后得到的闪式提取工艺参数可用于板蓝根药材的提取。

Objective： To optimize the flash extraction process of Isatidis Radix by Box-Behnken experimental design. Methods： A flash extraction method was used with the extraction voltage, material-liquid ratio and extraction time as the independent variables. A Hassan＇ s method was used to calculate the normalized value （OD） of comprehensive evaluation of the dry extract yield and the epi- goitrin extraction rate in order to establish the mathematical relationship between the comprehensive evaluation OD and the independent variables, and the response surface method was used to predict the best process conditions. Results： The optimum process conditions were as follows： voltage of 88 V, material-liquid ratio of 19.62, and extraction time of 2.03 min. The rate of dry extract and epigoitrin extraction was 37. 902% and 0. 1887%, respectively. Conclusion： The measured value is close to the predicted one,which indicates the comprehensive flash extraction parameters optimized by Box-Behnken experimental design can be used for the extraction for Isatidis Radix.