HPLC法测定硝酸甘油软膏的含量

Determination of Nitroglycerin Ointment by HPLC

目的:优化和完善硝酸甘油软膏的含量测定方法。方法:采用高效液相色谱法,色谱柱为Hypersil ODS(150 mm×4.6 mm,5μm)柱,流动相为乙腈∶水(50∶50),检测波长为220 nm,流速为1 ml·min^(-1),柱温为30℃,进样量为20μl。结果:硝酸甘油在0.020 3~0.203 3 mg·ml^(-1)浓度范围内呈现良好的线性关系(r=0.999 9),平均加样回收率为99.70%(RSD=1.06%,n=9)。结论:该方法快速、准确、重复性好,可用于硝酸甘油软膏中硝酸甘油的含量测定。

Objective： To optimize and improve the content determination method for nitroglycerin ointment. Methods： An HPLC method was used, the column was Hypersil ODS （150 mm ×4.6 mm, 5 μm） , the mobile phase was acetonitrile ： water （50 ： 50 ） , the detection wavelength was set at 220 nm,the flow rate was 1 ml min-1 ,the column temperature was 30℃ ,and the injection volume was 20 μl. Results： The results showed a good linear relationship within the concentration range of 0. 020 3-0.203 3 mg ml-1 （r = 0. 999 9 ）, and the average recovery was 99.51% （RSD = 1.06% , n = 9 ）. Conclusion： The method is rapid, accurate and reproduci- ble, and can be used to determine the content of nitroglycerin in nitroglycerin ointment.