质谱快速分析猪肉中痕量沙丁胺醇及克伦特罗

Rapid Analysis of Trace Salbutamol and Clenbuterol in Pork Samples by Mass Spectrometry

采用内部萃取电喷雾电离质谱（iEESI-MS）技术,在无需样品预处理的前提下,采用标准加入法直接对猪肉组织中沙丁胺醇与克伦特罗进行定性和定量分析。结果表明,本实验对猪肉组织中沙丁胺醇与克伦特罗具有较高的灵敏度,单个样品单一指标的检测时间少于30 s。在0.01~1000μg/kg浓度范围内,信号强度对数（Y）与浓度对数（X）具有较好的线性关系,定量限分别为6.2和9.8 ng/kg。本方法分析速度快、样本耗量少、灵敏度高,适用于猪肉中痕量沙丁胺醇与克伦特罗等＂瘦肉精＂的快速检测。

Salbutamol and Clenbuterol are often used as β-agonists and illegally added in pig feed,resulting in pork contaminated and even leading athletes excessive excitability. Therefore,it has great significance for establishing a new rapid detection method of salbutamol and clenbuterol in pork. In this study,internal extraction electrospray ionization-mass spectrometry（iEESI-MS）technology was used for directly qualitative and quantitative analysis of salbutamol and clenbuterol in pork tissues without sample pretreatment. The results showed that this method had a high sensitivity to salbutamol and clenbuterol analysis with detection limits of（LOD）6. 2 and 9. 8 ng / kg,respectively,while the analysis time for detecting single sample and single index was less than 30 s. In a concentration range of 0. 01-1000 μg / kg,the logarithm of signal intensity（Y）and the logarithm of concentration（X）have a good linear relationship. This method has many advantages such as rapid analysis,low sample consumption and high sensitivity,which is ideal for rapid detection of trace salbutamol and clenbuterol.