溶血和乳糜血对PT和APTT测定干扰的评价

Evaluation the interference of PT and APTT on hemolysis and chyle blood

目的依据美国临床实验室标准化协会(CLSI)EP7-A2文件评价溶血和乳糜血对凝血酶原时间(PT)和活化部分凝血活酶时间(APTT)测定的干扰。方法按照"配对差异"方案,配置含不同浓度血红蛋白(Hb)和不同浊度的乳糜的正常及高值PT和APTT标本,进行干扰评价试验。结果正常水平的PT和APTT不受Hb和乳糜浊度的干扰。当Hb≥2.55g/L时,Hb对高值APTT测定产生负干扰,并呈线性函数关系,Hb≥10.20g/L时对高值PT有干扰;当乳糜浊度≥745FTU时,乳糜血对高值PT测定产生正干扰,呈线性函数关系;高值APTT测定不受乳糜血干扰。结论中度溶血对高值APTT干扰明显,重度溶血对高值PT测定有干扰;中度乳糜血对高值PT干扰明显;正常水平的PT和APTT不受溶血和乳糜血的干扰,高值APTT不受乳糜血干扰。

Objective To investigate the interference effect of prothrombin time（PT）and actived partial thromboplastin time（APTT）on hemolysis and chyle blood according to EP7-A2 document from the Clinical Laboratory Standards Institute（CLSI）.Methods Different concentrations of hemoglobin（Hb）and chyle（Chy）plasma of normal and high levels prothrombin（PT）and activated partial thromboplastin time（APTT）were tested according to the ＂paired difference＂ scheme.Results The normal level of PT and APTT revealed insignificantly interference by Hb or Chy.A negative interference and a linear function relationship were found between Hb and high level of APTT,especially when Hb≥2.55g/L,However,this circumstances had happened in the high level of PT only when Hb≥10.20g/L.Besides,a significant positive interference and a linear function relationship between Chy and high level of PT were found,especially when Chy≥735FTU.Interestingly,no significant interference effect was found between high level of APTT and Chy.Conclusion Moderate hemolysis has a significant interference of high level APTT.Severe hemolysis and moderate Chy interference the high value of PT obviously Normal level of PT and APTT showed on interference effect on hemolytic and Chy.Meanwhile,the high level of APTT showed on interference effect on Chy.