耳聋丸中6种成分的HPLC-波长切换法测定

Determination of Six Compounds in Erlong Pills by HPLC with Wavelength Switching

建立了高效液相色谱法测定耳聋丸中6种成分的方法。采用Dikma Platisil ODS色谱柱,以乙腈∶0.1%磷酸为流动相,梯度洗脱,切换波长检测,检测波长分别为240 nm(京尼平龙胆双糖苷、栀子苷、木通苯乙醇苷B)、274 nm(龙胆苦苷、黄芩苷、甘草酸铵)。上述6种成分均达到基线分离,在相应范围内线性关系良好,平均回收率为97.5%~104.6%,RSD为1.15%~1.83%。

An HPLC method was established for the determination of six main components in Erlong pills. A Dikma Platisil ODS column was used, with the mobile phase of acetonitrile ： 0.1% phosphoric acid by gradient elution. The detection wavelength was switched from 240 nm for genipin 1-gentiobioside, geniposide and calceolarioside B to 274 nm for gentiopicrin, baicalin and ammonium glycyrrhizinate. The results showed that all the six components were well separated, and the linear relationships were good in their corresponding ranges. Their average recoveries were 97.5 %-104.6%, with RSDs of 1.15 %-1.83 %.