摘要
目的:观察竹叶黄酮(bamboo leaf flavone)对糖尿病大鼠认知功能障碍的影响及其相关机制的研究。方法:40只雄性SD大鼠高糖高脂喂养4周后,采用链脲佐菌素(streptozocin,STZ)一次性腹腔注射(50 mg/kg)诱导大鼠Ⅱ糖尿病模型。造模成功的36只大鼠随机分为三组:模型组(DM组)、模型+给药组(DM+BLF组)、模型+溶剂对照组(DM+CO组),每组12只。同时,随机选取12只同月龄正常的大鼠作为对照组(CON组)。给药12周后,通过水迷宫(morris water maze,MWM)测试,观察竹叶黄酮对糖尿病大鼠认知障碍的影响;采用尼氏染色法观察竹叶黄酮对糖尿病大鼠海马神经细胞的影响;同时应用免疫印迹(weatern-blot,WB)法检测竹叶黄酮对糖尿病大鼠海马区白介素-6(interleukin-6,IL-6),环氧合酶-2(cyclooxygenase-2,COX-2)和肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)等炎症因子表达的影响。结果:竹叶黄酮可以减轻糖尿病大鼠认知功能障碍,保护海马区神经元,抑制糖尿病大鼠海马区炎症因子IL-6、COX-2和TNF-α的蛋白表达。结论:竹叶黄酮降低海马神经元损伤,抑制大鼠脑海马的炎症反应改善糖尿病大鼠认知功能障碍。
Ojeive: o udy he effe of moo Lef Flvone on ogniive defii in diei nd he elevn mehnim.Mehod: ol of 40 mle D wee fed wih high gluoe nd high f die fo 4 week, hen wee eeived ingle inpeionel injeion of epozooin ( Z, 50 mg/kg ) o indued diei mie.36 who wee ueful, model wee divided ino hee goup: DM goup, DM+LF goup nd DM+O goup, 12 in eh goup.Menwhile, 12 ge-mh wee eleed ON goup.fe moo Lef Flvone wee eed wih 12 week, Moi we mze ( MWM ) w ued o evlued he effe of moo Lef Flvone on he ogniive defii in diei .Nil' ining w ued o deeed he impliion of moo Lef Flvone in hippompl neuonl dmge in diei .Moeove, we ued W mehod o eed he expeion of Ineleukin-6 ( IL-6 ), "ylooxygene-2 ( OX-2 ) nd umo neoi fo- α( NF- α) in hippompu of eh goup.eul: moo Lef Flvone ould melioe ogniive defii, edue he hippoempu neuonl dmge nd inhii he expeion of inflmmoy fo in diei .onluion: moo Lef Flvone llevie ogniive defii nd poe hippompl neuon in diei hough inhiiing inflmmion.
出处
《中国医学创新》
CAS
2016年第35期21-23,共3页
Medical Innovation of China
基金
国家自然科学基金(81371300)
关键词
竹叶黄酮
糖尿病大鼠
认知障碍
海马
炎症
Bamboo Leaf Flavone
Diabetic rats
Cognitive deficit
Hippocampal neuron
Inflammation