摘要
目的:探讨利用HPV E6/E7 m RNA检测技术检测妇科子宫颈上皮内瘤变的临床应用价值。方法:对69例TCT检查结果为宫颈上皮内瘤变的患者,应用HPV E6/E7 m RNA检测技术对感染HPV病毒中的癌基因片段E6、E7转录子做出定量检测。结果:>ASC-H组E6/E7 m RNA转录阳性率、转录数分别为60.6%、(18 285.42±1487.71),均高于≤ASC-H组的33.3%、(2668.43±681.39),差异均有统计学意义(P<0.05);宫颈细胞学检查为宫颈癌的HPV E6/E7 m RNA转录数明显高于其他类型,差异有统计学意义(P<0.05)。结论:宫颈上皮内瘤变与E6/E7 m RNA转录具有相关性,即转录数与病变的进展程度及细胞的恶性转化密切相关,在宫颈癌筛查中检测HPV E6/E7 m RNA的转录具有潜在价值。
Objective： To discuss the clinical application value of quantitative detection the HPV E6/E7 mRNA in cervical intraepithelial neoplasia.Method： In 69 cases of TCT examination results for cervical intraepithelial neoplasia, use of HPV E6/E7 mRNA detection technology for the infection of HPV virus in the cancer gene fragment E6, E7 transcription to make quantitative detection.Result： 〉ASC-H group HPV E6/E7 mRNA transcription positive rate （ 60.6% ） was higher than the ~〈 ASC-H group （ 33.3% ）, 〉ASC-H group HPV E6/E7 mRNA transcription number （ 18 285.42 ＋ 1487.71 ） was higher than the 〈~ASC-H group （ 2668.43 ~ 681.39 ）, the differences were statistically significant （ P〈0.05 ） .Cervical cytological examination for cervical cancer HPV E6/E7 mRNA transcription number was significantly higher than other types, the difference was statistically significant （P〈0.05） .Conclusion： The cervical intraepithelial neoplasia is closely related to the E6/E7 mRNA transcription, that is, and that means the HPV E6/E7 mRNA transcription affect the the progression of lesions and malignant transformation of cells, quantitative detection the HPV E6/E7 mRNA has potential clinical application value in the screening of cervical intraepithelial neoplctsia.
出处
《中国医学创新》
CAS
2016年第36期98-101,共4页
Medical Innovation of China
基金
内蒙古自治区高等学校科学研究项目(NJZY13246)
