多肽微阵列技术在自身免疫性甲状腺疾病诊断中的初步应用

Peptide Microarray Platform Based on iPDMS for Autoimmune Thyroid Disease

目的建立多肽微阵列的方法用于自身免疫性甲状腺疾病的诊断。方法以自主研制的改性硅胶膜（iPDMS）为固体支撑物材料，以促甲状腺激素受体及甲状腺过氧化物酶为对象，将其进行叠瓦式切割，制备多肽芯片，对甲状腺患者血清进行检测。结果多肽芯片对抗促甲状腺素受体抗体阳性和抗甲状腺过氧化物酶抗体阳性甲状腺患者血清具有良好响应。结论基于iPDMS固体支撑材料的多肽芯片在甲状腺疾病诊断和鉴别诊断中具有较好应用前景，其应用有可能实现AITD实验诊断从单指标检测转变为“多指标联合检测型”。

Objective To establish peptide microarray applied to diagnosis of thyroid disease. Melhods 76 and 93 peptides （20aa） derived from thyroid stimulating hormone receptor （TSHR） and thyroid peroxidase （TPO）, respectively,which were syn thesized and spotted on our chip in the form of microarray. The chip is based on our self-developed initiator integrated Polydimethyl- siloxane （iPDMS） and applied in patients with thyroid disease. Results Peptide microarray exhibited signal response to serum from the patient with positive anti-TSHR antibodies （TRAb） or anti-TPO antibody （TPOAb）. The TRAb and TPOAb-bound peptide repertoires were constructed,respectively. Conclusion Peptide microarray platform based on iPDMS could be applied in diagnosis of thyoid disease, which is expected to achieve the thyroid disease clinical laboratory diagnosis from the single indicators into the era of multiple markers.