哮喘和COPD患者急性发作期痰中细胞因子IL-5、IL-13、TNF-α测定的临床意义

Clinical significance of IL-5, IL-13, TNF-c~ determination in sputum of patients with acute exacerbation of asthma and COPD cytokines

目的探讨哮喘和慢性阻塞性肺疾病（COPD）患者急性发作期时痰中细胞因子白介素5（IL-5）、白介素13（IL-13）、肿瘤坏死因子d（TNF—α）的水平及其测定的临床意义。方法采用酶联免疫法和放射免疫方法对17例COPD患者、19例哮喘患者和12例健康者痰液中IL-5、IL-13和TNF—α的水平进行检测。结果哮喘急性发作期痰中IL-5、IL-13、TNF-α含量显著高于健康者（P〈0．05）。COPD患者急性发作期痰中IL-5、IL-13、TNF—α含量显著高于健康者（P〈0．05）。哮喘组痰液中IL-5、IL-13水平显著高于COPD组（P〈0．02，P〈0．05），而TNF-α水平在两患病组中无明显差异（P〉0．05）。结论IL-5、IL-13和TNF-α在哮喘发病过程中起重要作用。IL-5、IL-13和TNF-α共同参与了COPD气道炎症反应。

Objective To investigate the concentration of IL-5,IL-13 and TNF-α in sputum during acute episods in patients with COPD or Asthma and that in healthy peoples＇ sputum. Methods Collected sputum from 19 asthma,17 COPD and 12 healthy con- trois. IL-5,IL-13 and TNF-α were measured by enzyme-linked immunoassay （ELISA） and radioimmunoassay technique（RIA）. Results At acute attack asthmatic stage,the sputum levels of IL-5 ,IL-13 ,TNF-α of patients with were significantly higher than these eytokines （P〈0.05） in normal eontrols. At acute stage of COPD,IL-5 and IL-13 concentration were higher than which form normal controls （P〈0.05）. Conclusion IL-5,IL-13 and TNF-α play an important role in asthma. IL-5,IL-13 and TNF-α took part in the pathogenetic process of COPD.