HE切片褪色后行EBER原位杂交检测方法的探讨

Study on the technique of EBER in situ hybridization on HE staining sections after decoloration

目的探讨将HE切片褪色后行EB病毒编码的小RNA(EBER)原位杂交检测的方法。方法收集40例EBER原位杂交检测阳性病例的HE切片作为实验组,相同蜡块的连续切片作为对照组,分别进行EBER原位杂交检测。结果实验组中,38张切片的组织结构全部完整,2张骨髓穿刺切片中的骨小梁脱片,但剩余组织组织结构保持完整。所有40张切片均杂交阳性,阳性信号位于细胞核,呈棕黄色,定位准确、清晰可读,杂交背景干净。阳性细胞数目及强度与对照组无差别。结论将HE切片褪色后行EBER原位杂交检测,方法简单、可靠,在病理诊断中有一定的应用价值。

Objective To explore the technique of EBER in situ hybridization on HE staining sections after decoloration. Methods Forty cases of EBV- positive expression were tested in this study. One HE section and one sequential section cut from the same tissue block of each case had been allotted into two groups： trial group（ HE sections） and control group（ sequential sections）. In situ hybridization had been performed in both groups respectively. Results In trial group,38 out of 40 sections showed intact tissue structure while on the left 2 sections of bone marrow biopsy,bone trabecula detached from the slide. But all these 40 sections were positive for EBER in situ hybridization. The positive signal was located at the nucleus as brownish yellow. The signal location was accurate and coloring results were discernible. No background interruption had been detected. Conclusion The technique tried in this study is simple,reliable and applicable for pathological diagnosis.