响应面法优化生姜水溶性膳食纤维提取及抗氧化活性研究

Study on Optimization of Soluble Dietary Fiber Extraction by Water Response Surface Method and Antioxidant Activity of Ginger

试验采用超声协同酶法,研究提取生姜中水溶性膳食纤维的影响因素,通过响应面法优化提取工艺条件,并对其抗氧化活性进行探讨。试验结果表明,最佳工艺条件为加酶量3.5%,超声时间15 min,超声功率150 W,生姜中水溶性膳食纤维最高的提取率为11.30%±0.36%。生姜中水溶性膳食纤维对·OH、O_2^-·具有一定的清除能力,对DPPH具有较强的清除能力,且均随着SDF浓度增加清除率逐渐增大,但同VC的清除效果相比较,均明显低于VC。

Ultrasonic-enzymatic extracting methods were used to extract water-soluble dietary fiber of ginger. The extraction conditions were optimized by response surface method and anti-oxidative activity was also discussed. The results showed that the optimal extracting conditions were enzyme volume 3.5%, ultrasonic time 15 min, ultrasonic power 150 W. The highest extracted rate of water-soluble dietary fiber of ginger was 11.30%±0.36%. Soluble dietary fiber of ginger was characterized by certain ability to scavenge ·OH and O2· and higher ability to scavenge DPPH. With the increase of SDF concentration, the clearance rates increased gradually. But the clearance rates were lower than that of VC.