摘要
通过转基因技术改良植物品质近几年已成为热点研究问题,基因工程不断发展,单基因转化技术已不能满足人们对植物改良的需要。更多的研究者投身于参与某个代谢途径的多个基因在植物体中共同表达的研究,通过多基因调控来获得更好的植物性状。基因的协调表达有四种研究思路,在此基础上多基因转化方法可概述为传统转化法、改进后的共转化法,及新兴的基因融合方法,综合分析每种方法在植物代谢调控中的优缺点与应用,并探讨多基因整合的不稳定及相互作用问题。
Improving plant quantity traits through transgenic technology has been increasingly concerned in modern genetic transformation field. With the rapid development of genetic engineering,single gene transformation technology is insufficient for the plant modifications. In order to get better plant traits by multigene regulation,more researchers have focused on the study of genetic transformation,making the genes involved in metabolic pathways coexpression in plants. There are four genome expression routes for genes selection and basically plant genetic transformation methods cover the traditional and improved transformation methods,emerging polygene fusion. The advantages and disadvantages of each method in plant metabolism regulation and application progress were analyzed comprehensively and the future development was discussed.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2016年第12期111-116,共6页
China Biotechnology
基金
国家自然科学基金资助项目(31271959)
关键词
植物
多基因转化
传统转化
基因融合
Plants
Multiple gene transformation
Traditional transformation
Polygene fusion