摘要
目的探讨胸腔积液腺苷脱氨酶(ADA)、γ-干扰素(IFN-γ)、肿瘤坏死因子-α(TNF-α)与胸腔积液沉渣DNA(TBDNA)联合检测在结核性胸膜炎诊断中的价值。方法选取2014年5月至2015年11月经诊断为结核性胸腔积液的62例患者作为结核性胸腔积液组,另选取62例非结核性胸腔积液的患者作为对照(非结核组为恶性胸腔积液),采用酶比色法检测ADA活性,采用酶联免疫吸附试验(ELISA)检测IFN-γ和TNF-α,采用PCR定性检测TB-DNA。计算4项指标单独检测和联合检测结核性胸腔积液的敏感性和特异性。结果结核性胸腔积液组的ADA、IFN-γ和TNF-α水平均明显高于非结核性胸腔积液组(P<0.05)。ADA、IFN-γ、TNF-α和TB-DNA对结核性胸腔积液组的单独检测阳性率均明显高于非结核性胸腔积液组的检测阳性率(P<0.05)。4项指标联合检测结核性胸腔积液组的阳性率显著高于非结核性胸腔积液组(P<0.05)。4项指标联合诊断结核性胸膜炎的敏感性和特异性分别为91.9%和93.5%,均显著高于各指标单独诊断的敏感性和特异性。结论联合检测胸腔积液中ADA、IFN-γ、TNF-α和TB-DNA能够提高诊断的准确性,可以作为早期诊断与鉴别结核性胸膜炎的重要参考指标。
Ojeive o udy he vlue of omined exminion of D, IFN-γ,NF-α nd pleul effuion edimen DN (-DN) fo dignoi of ueulou() pleuii. Mehod ol of 62 e of pleul effuion in ou hopil fom My 2014 o Noveme 2015 wee eleed he pleul effuion goup. Ohe 62 e of non- pleul effuion wee ele- ed he onol goup( mlignn pleul effuion). he D iviy w deeed y enzyme oloimeie y. IFN-y nd NF- wee deeed y ELI. -DN w quliively deeed y P. he eniiviy nd peifiiy of ingle deeion nd om- ined deeion of hee 4 indexe fo pleul effuion wee luled. eul he level of D,IFN-γ nd NF-α of he pleul effuion goup wee ignifinly highe hn hoe of he non- pleul effuion goup(P〈0.05). he poiive deeion e of D,IFN-γ,NF-α nd -DN fo ingly deeing in he pleul effuion goup wee ignifinly highe hn hoe of he non- pleul effuion goup(P〈0.05). he poiive deeion e of 4-indio omined deeion in he pleu- l effuion goup w ignifinly highe hn h in he non- pleu effuion goup. he eniiviy nd peifiiy of 4-index omined deeion fo dignoing pleuiy wee 91.9% nd 93.5% epeively, whih wee ignifinly highe hn h of eh index fo ingly dignoing pleuiy. onluion he omined deeion of pleul effuion D,IFN-γ,NF-α nd - DN n impove he uy of dignoi,nd n e ued he impon efeene indexe fo he ely dignoi nd diffeen- il dignoi of pleuiy.
出处
《检验医学与临床》
CAS
2017年第1期24-26,共3页
Laboratory Medicine and Clinic
基金
2014年大连市医学卫生科学研究计划项目