低温冷冻保存对人牙本质和牙髓细胞生物学性能影响的初步研究

Studies on the effect of cryopreservation on the biomechanical characteristics of human dentin andpulp cells

目的:比较人离体牙在低温冷冻保存前后的牙本质和牙髓细胞的生物特性,探讨其应用价值。方法:收集需要拔除的无龋坏、无缺损新鲜离体前磨牙或第三磨牙40颗。其中20颗离体牙制成牙本质盘标本,随机分为程序降温组和新鲜拔除组,每组10颗;冻存复苏后,进行力学分析以及用扫描电观察两组标本的牙本质表面和纵剖面。剩余20颗牙使用改良组织块法原代分离牙髓细胞,冻存复苏后,观察分析冻存前后的细胞形态及生长特性。结果:低温冷冻保存复苏后,离体牙的牙本质和牙髓细胞的生长特性的情况均无明显差异。结论:应用程序低温冷冻技术保存牙齿,牙体牙髓组织生物学特性均能保持良好。

Objective：Studies on the effect of cryopreservation on the biological characteristics of dentine and dental pulp cells in vitro.Methods：A total of 40 fresh teeth（the first premolar or second premolar） were collected and respectively experiment with dentine mechanical morphological analysis and dental pulp cells growth experiments.At the experiment of dentine mechanical morphological analysis,20 fresh extracted human premolars were selected,prepared into dentin discs,and randomly divided into 2 groups（group A：fresh teeth group,group B：programmed cooling group）,which were measured for compression strength and scanning electron microscope analysis.Another 20 teeth,using improved tissue block method of separation of dental pulp cells,the original generation of cryopreserved after recovery were observed before and after analysis of cryopreserved cells morphological and growth characteristics.Results：There were no obvious differences between groups with cryopreservation in growth characteristic of dentine and dental pulp cells.Conclusions：Tooth pulp tissue can keep good biological characteristics with cryopreservation.