膨体聚四氟乙烯植入后感染与毛囊的相关性研究

Correlation Analysis Between Infection and Hair Follicles after Expanded Polytetrafluoroethylene Implantation

目的观察膨体聚四氟乙烯(e-PTFE)植入后感染与毛囊的关系,探讨植入感染的可能原因。方法将40只SD大鼠随机分成实验组(n=20)和对照组(n=20)。大鼠背部左右两侧,实验组分离皮下层(A组)及真皮下层(B组),分别植入假体;对照组左、右两侧(C组、D组)仅分离相应层次。于术后1、3、4周取材,大体及组织学观察样本局部情况,ELISA检测相关炎症因子的变化,并进行统计学分析。结果 B组可见较明显的皮肤红肿、局部渗出,并且随时间延长而加重,最后出现皮肤坏死,其余各组均无上述现象。组织学及ELASA结果提示,B组炎症细胞及炎症因子均较A组严重,且严重程度随时间延长而增加。C、D两组均无上述变化。相关差异具有统计学意义。结论膨体聚四氟乙烯在大鼠真皮下层与毛囊根部接触,是引起植入后易发生感染的重要诱因之一。

Objective To investigate the correlation between infection and hair follicles after expanded polytetrafluoroethylene implantation, and to discuss the cause of infection. Methods Forty SD rats were randomly divided into the experimental group （n =20） and the control group （n =20）. In experimental group, e-FFFE was implanted subcutaneously （defined as group A） and subcorium （defined as group B） on the left and right side of the rats＂ back respectively. In control group, the left side （defined as group C） and the right side （defined as group D） of the rats＂ back were only separated anatomically. Samples were harvested at 1, 3 and 4 weeks post-operation. Samples were observed grossly and histologically. The corresponding inflammatory factors were detected via ELISA and statistical analysis was done. Results Swelling skins with effusion were found in group B, which got worse gradually. However, no similar symptom occurred in any other groups. Both histological and ELISA analysis indicated that group B undergone more severe inflammatory reaction than group A. Also the situation worsen throughout observation time. No inflammatory reaction was found in group C and D. The relevant differences were statistically significant. Conclusion E-PTFE contacted with hair follicles could lead to serious inflammation, and it is one of the important causes of local infection after rhinoplasty with e-PTFE.