光谱能量分布对大豆胚尖再生体系的影响

Effects of spectral energy distribution on soybean embryonic tip regeneration system

[目的]本文旨在研究不同光谱能量分布对大豆胚尖再生体系的影响。[方法]以浸泡24 h的大豆胚尖为材料,依次接种于丛生芽诱导培养基、伸长培养基和生根壮苗培养基中,试验的光谱组合处理为:80%红光(660 nm)+20%蓝光(R_(660)B)、80%红光(630 nm)+20%蓝光(R_(630)B)、66.8%红光(660 nm)+16.6%蓝光+16.6%黄光(R_(660)BY)、66.8%红光(630 nm)+16.6%蓝光+16.6%黄光(R_(630)BY)、66.8%红光(660 nm)+16.6%蓝光+16.6%绿光(R_(660)BG)、66.8%红光(630 nm)+16.6%蓝光+16.6%绿光(R_(630)BG),以荧光灯(W)为对照。光密度设置为50μmol·m^(-2)·s^(-1),光/暗时间为12 h/12 h。[结果]复合光谱中660 nm红光有利于丛生芽的诱导和伸长,促进再生苗的干质量和根系活力的增加以及气孔的发育。630 nm红光有利于再生苗根系的伸长和叶绿素的积累。在红蓝光的基础上添加黄光有利于丛生芽芽数的增加,促进再生苗主根直径、根系活力、干质量的增加,以及气孔的发育。添加绿光则显著促进气孔频数的增加。[结论]复合光谱有利于大豆胚尖丛生芽的诱导和再生苗的生长,R_(660)BY处理可以作为大豆胚尖再生体系的适宜光谱处理。

[ Objectives ] The aim of this study is to study the effects of spectral energy distribution on soybean embryonic tip regeneration system. [ Methods ] The soybean embryonic tips soaked for 24 hours were used as the experimental material and inoculated onto shoot initiation, shoot elongation and rooting mediums successively. Treatments in the experiment were ： 80% red light （ 660 nm） ＋ 20% blue light（ R660B）, 80% red light（630nm） ＋20% blue light（ R630B） , 66.8% red light（ 660 nm） ＋ 16.6% blue light＋ 16.6% yellow light （ R660 BY）, 66.8% red light （ 630 nm） ＋ 16.6% blue light ＋ 16.6% yellow light （ R630 BY）, 66.8% red light （ 660 nm） ＋ 16.6% blue light＋16. 6% green light（ R660BG）, 66.8% red light （630 nm） ＋ 16.6% blue light＋ 16.6% green light（ R630BG）, and fluorescent white lamps（W） were used as a control. The photosynthetic photon flux density（PPFD） was 50 μmol·m-2· s-1, and photoperiod was 12 h light/12 h dark. [ Results] The results showed that the treatments containing 660 nm red light contributed to the induction and elongation of the multiple shoot clumps from embryonic tips of soybean, and improved the accumulation of dry matter, root activity and the development of stoma of the regenerated plantlet. The treatments containing 630 nm red light contributed to the elongation of roots and the accumulation of chlorophyll content. The addition of yellow light to the complex spectra of red and blue light resulted in the increase of multiple shoot clumps, and improved the accumulation of dry matter, the diameter of the main roots, root activity and the development of stoma of the regenerated plantlet. The addition of green light to the complex spectra of red and blue light increased the stomata of frequency. [ Conclusions ] The complex spectra energy distribution contributed to the induction of the multiple shoot clumps and the growth of the regenerated plantlet. The R660BY treatment was the most suitable light treatment for the regeneration system of soybean embryonic tip.