抗脂肪细胞膜蛋白抗体对试验性肥胖大鼠5种激素水平的影响

Effects of Antibody against Adipocyte Membrane Protein on Five Kind of Hormone Levels in Experimental Obese Rats

为了验证抗脂肪细胞膜蛋白抗体减少动物脂肪沉积的作用效果,并对该抗体用于人类肥胖的治疗提供理论依据和试验资料,本试验分别对4组试验性肥胖大鼠注射0、0.1、0.5、1.0mg/mL剂量的抗脂肪细胞膜蛋白抗体,并检测4周内大鼠血清中5种激素含量及变化。结果显示,试验组血清中胰岛素(INS)含量低于对照组,Ⅰ组与Ⅳ组4周内均差异显著(P<0.05);试验组与对照组、胰岛素样生长因子1(IGF-1)含量无显著差异(P>0.05);注射抗体后,试验组生长激素(GH)含量显著增高,且Ⅰ、Ⅲ组4周内均差异显著(P<0.05);第1周内,试验组三碘甲状腺原氨酸(T3)含量与对照组无显著差异(P>0.05),后3周内Ⅰ组和Ⅳ组差异显著(P<0.05);第1、3、4周内,Ⅰ组和Ⅳ组四碘甲状腺原氨酸(T4)含量差异显著(P<0.05),第2周内无显著差异,Ⅱ组和Ⅲ组差异均不显著(P>0.05)。此外,不同组间的差异显著性随注射剂量不同而不同。

To verify the effects of antibody against adipocyte membrane protein on animal fat deposition and provide the theoretical basis and experimental data for using the antibody to reduce human obesity, sixty experimental obese rats were injected with antibodies at 0,0.1,0.5,1.0 mg/mL respectively and the serum hormone contents were determinated every week. The results showed that the INS content in treatment groups was lower than that in the control group,but there was significant difference between Ⅰ group and Ⅳ groups（P〈0.05）.The content of IGF-1 showed no significant difference between treatment groups and control group （P〈0.05）.The GH contents in treatment groups increased significantly compared with the control group,and there was significant difference between Ⅰ group and Ⅲgroup at 4 week （P〈0.05）.In the first week, the contents of T3 in treatment groups had no significant difference with control group （P〉0.05）. But it showed significant difference between Ⅰ and Ⅳ groups during the next 3 weeks （P〈0.05）. The T4 contents in Ⅰ and Ⅳ groups had significant difference at 1st, 3rd and 4th week （P%0.05）,and the Ⅱ group and Ⅲ group had similar tendency at 4 weeks（P〈0.05）.In conclusion,the hormones among different groups varied with the dose of antibody injected.