丛台酒大曲中高产淀粉酶细菌的分离和鉴定

Isolation and Identification of a Bacteria Strain with High Yield of Amylase from Congtai Daqu

为从丛台酒中温大曲中分离高产淀粉酶的菌株,通过透明圈法和淀粉酶活力测定分别进行了初筛和复筛,并通过生理生化性能鉴定确定高产淀粉酶细菌的分类。经筛选得到的高产淀粉酶细菌C3,其淀粉酶活力为63.1 U,比原厂浓香型大曲提高了44.4%。经鉴定,C3为革兰氏阳性芽孢杆菌,其菌落为黄褐色,表面无光泽、不透明且边缘整齐,有不规则突起。对丛台酒高产淀粉酶细菌的分离,将为酒厂提高大曲质量和出酒率提供菌种和依据。

In this study, transparent circle method and amylase activity measurement were adopted respectively for primary screening and secondary screening of bacteria strains with high yield of amylase from Congtai Daqu. As a result, strain C3 with high yield of amylase was obtained and its amylase activity reached up to 63.1 U, 44.4 % higher than that of the original Daqu. Through physiological and physiochemical identification, strain C3 was identified as gram-positive Bacillus and its single colony was yellowish-brown with matt surface, neat edge and irregular convex. This study provided reference for obtaining useful bacteria strains in distilleries to improve Daqu quality and liquor yield.