根际低氧对不同抗性牡丹植株呼吸代谢的影响

Effect of root zone hypoxia stress on respiratory metabolism between two Paeonia suffruticosa varieties

以牡丹品种"洛阳红","胡红"为试材,研究了根际低氧胁迫下两牡丹品种呼吸代谢应答机制及差异。结果表明,胁迫前期根系苹果酸脱氢酶(MDH)活性降低;丙酮酸脱羧酶(PDC),乙醇脱氢酶(ADH)和乳酸脱氢酶(LDH)活性前期升高,后期降低;葡萄糖-6-磷酸脱氢酶(G-6-PDH)、乳酸、乙醇、乙醛及丙酮酸含量显著增加。根PDC、ADH和LDH活性最大值时,"洛阳红","胡红"根PDC、ADH和LDH活性分别比各自对照增加103.1%,76.3%;125.6%,91.3%;238.6%,157.7%。乳酸,乙醛及丙酮酸含量分别比各自对照增加151.1%,119.6%;284.8%,421.5%;75.8%,105.6%。表明根际低氧胁迫时"洛阳红"比"胡红"具备更完善的无氧呼吸代谢调节机制,更耐低氧。

Two Paeonia suffruticosa varieties‘Luoyang Hong＇and ‘Hu Hong＇were used as test materials in this study. The rhizosphere respiration metabolism and the difference between them were researched under root zone hypoxia stress. The results indicated that stress root MDH（ malic dehydrogenase） activity decreased; PDC（ pyruvate decarboxylase）,ADH（ alcohol dehydrogenase） and LDH（ lactate dehydrogenase） activity increased in the early days,the latter reduced. G- 6- PDH,lactic acid,ethanol,of acetaldehyde and pyruvate content increased significantly. Compared with the respective control,the maximum value of root PDC,ADH and LDH ‘Luoyang Hong＇and‘Hu Hong＇were increased by 103. 1% and 76. 3%,125. 6% and 91. 3%,238. 6% and 157. 7% respectively. Contents of lactic acid,acetaldehyde and pyruvic acid were increased by 151. 1% and 119. 6%,284. 8% and421. 5%,75. 8% and 105. 6% respectively. The results showed under root zone hypoxia stress,‘Luoyang hong＇had better anaerobic respiration adjustment mechanism than ‘Hu Hong＇and was more resistant to hypoxia.