偏肿革裥菌产漆酶活性的诱导

Induction on Laccase Activity Produced by Lenzites gibbosa

【目的】研究不同诱导剂及其添加量对漆酶的诱导效果,以期在漆酶活性优化培养的基础上进一步提高偏肿革裥菌的漆酶活性。【方法】通过单项诱导剂筛选试验,检测在液体培养条件下12种不同诱导剂对漆酶活性的影响,进行最佳单项诱导剂浓度的单因素试验,2种最佳诱导剂组合、Cu2+浓度、接种量、培养方式对漆酶活性的影响试验。【结果】在初始培养基中含有0.2 mmol·L^(-1)Cu2+的情况下,生物诱导剂麦麸和化学诱导剂愈创木酚对漆酶的诱导效果好、酶活性高,二者的最适添加量分别为1.5%和1.00 mmol·L^(-1),单独添加时在13天酶活性分别达1 873.16和1 975.72 U·L^(-1),是初始产酶培养基酶活性的13.0和13.7倍;而2种诱导剂组合即同时添加1.5%麦麸与1.00 mmol·L^(-1)愈创木酚,比单独使用其中1种诱导剂的效果更好,在150 r·min^(-1)摇动培养条件下,第11天酶活性峰值可达3 594.46 U·L^(-1),是初始培养条件酶活性的24.9倍。【结论】2种诱导剂组合可大幅度提高偏肿革裥菌的漆酶活性,比培养条件优化对酶活性的提高更显著。

【Objective】In order to enhance laccase activity of Lenzites gibbosa significantly,induction effects of different inducers and their optimal levels were studied. 【Method 】The effects of 12 kinds of inducers on laccase activity was determined under liquid culture through single factor tests. Several single factor screening tests consisting of optimal inducer＇s concentration,2 inducers combination,Cu2 ＋concentration,inoculum volume,and static or shaking culture were conducted to gain further optimal conditions. 【Result 】The effects of biological inducer wheat bran and chemical inducer guaiacol on laccase production were better,and a high laccase activity was obtained when added 0. 2 mmol·L^-1Cu2 ＋,the optimum levels of them were 1. 5% and 1. 00 mmol·L^-1,respectively; when one of them alone added,L.gibbosa laccase activity would reached 1 873. 16 U·L^-1and 1 975. 72 U·L^-1,which were 13. 0 and 13. 7 times than the laccase activity of the initial medium,respectively. When 1. 5% wheat bran and 1. 00 mmol·L^-1guaiacol were added simultaneously,the laccase activity was higher than that of each one of them alone added,and the peak value of laccase activity would reached 3 594. 46 U·L^-1on 11 day under 150 r·min^-1shaking condition,which was 24. 9 times than that of the initial medium. 【Conclusion】Two inducers combination could enhance laccase activity substantially,the increase in laccase production induced by them was more obvious than that of culture condition optimization.