河南不同产地商陆药材质量分析

Quality of Phytolaccae Radix from Different Sources in Henan

目的:对河南不同产地商陆药材化学成分、酸不溶性灰分及指纹图谱进行分析,为商陆药材的开发利用、质量标准的制定提供参考依据。方法:采用薄层色谱法进行定性鉴定;HPLC-ELSD测定商陆皂苷甲含量,流动相甲醇-0.4%冰乙酸(30∶70),流速1.0 m L·min^(-1),柱温30℃,蒸发光散射检测器检测,气体流速2.0 L·min^(-1),漂移管温度90℃,记录时间20 min,进样量20μL。指纹图谱采用HPLC-ELSD法,梯度洗脱,气体流速2.0 L·min^(-1),漂移管温度105℃。结果:不同产地商陆中商陆皂苷甲含量存在差异,其中济源、西峡、鲁山产商陆中商陆皂苷甲的含量较高,在1.29%~3.52%。酸不溶性灰分均合格。指纹图谱标出9个共有峰,以峰7(商陆皂苷甲)为参照峰。结论:该研究结果可作为制定商陆质量标准的参考依据。

Objective： To provide a basis for the development,utilization and qualitycontrol of Phytolacca Radix through quality and fingerprint analysis of Phytolacca Radix from different sources in Henan.Method： Thin-layer chromatography was used for qualitative identification; HPLC-ELSD method was used for the determination of esculentoside A content with moble phase of methanol-4% acetic acid（ 30 ∶ 70）; the flow rate was1. 0 m L·min^-1 and column temperature was 30 ℃,evaporative light scattering detector（ ELSD） was used for detection： gas flow rate of 2. 0 L·min^-1,drift tube temperature of 90 ℃,record time of 20 min and the sample size of 20 μL. Fingerprint analysis was conducted by using HPLC-ELSD and gradient elution,with a gas flow rate of2. 0 L·min^-1,drift tube temperature of 105 ℃. Result： There was difference in content of esculentoside A of the samples from different areas,and the content was higher between 1. 29%-3. 52% in samples from Jiyuan,Xixia and Lushan; acid-insoluble ash content was qualified in all samples. There were 9 common peaks in the fingerprint,and peak 7 was the reference peak. Conclusion： The results of this study can be used as the reference for the formulation of Phytolaccae Radix quality standard.