结核潜伏感染蛋白Rv2657cT细胞和B细胞表位的预测与分析

Prediction and analysis of T-cell and B-cell epitopes of Mycobacterium tuberculosis Rv2657c protein

目的:预测结核分枝杆菌Rv2657c蛋白的抗原表位,了解其免疫原性。方法:应用DNAStar软件预测Rv2657c蛋白的T细胞和B细胞表位;Blast方法分析该蛋白氨基酸序列与人类蛋白序列的相似性;SYFPEITHI超基序法、BIMAS量化基序法及Net CTL法预测蛋白的CTL表位;RANKPEP及SYFPEITHI超基序法远程预测Th细胞表位。结果:DNAStar软件预测Rv2657c蛋白有5个B细胞抗原表位,6个T细胞抗原表位。SYFPEITHI超基序法、BIMAS量化基序法及Net CTL法预测该蛋白有6个CTL表位;RANKPEP及SYFPEITHI超基序法预测该蛋白有38个Th表位。结论:Rv2657c蛋白既有B细胞表位也有T细胞表位,可能成为结核病诊断抗原分子和候选疫苗抗原。

Objective To predict the epitopes of Mycobacterium tuberculosis Rv2657c protein, to understand its immunogenicity Methods The T-cell and B-cell epitopes of Mycobacterium tuberculosis Rv2657c protein were predicted by DNAStar software package. The homology of Rv2657c amino acid sequence with the human protein sequences was prepared using Blast method, then the CTL epitopes were predicted using SYFPEITHI supermotif method, BIMAS quantitative motif method and NetCTL prediction method, and the Th epitopes were predicted by RANKPEP and SYFPEITHI supermotif prediction method. Results The prediction using DNAStar software package showed that Rv2657c protein had 5 B-cell epitopes and 6 T-cell epitopes. The protein had 6 CTL epitopes and there were 38 Th epitopes. Conclusion Rv2657c protein has both B-cell epitopes and T-cell epitopes. It may be a candidate target antigen for the studies of vaccine and diagnosis of tuberculosis.