HPLC-ELSD法测定三种植物基原金线莲的金线莲苷含量

Content determination of kinsenoside in Jin-Xian-Lian from three Anoectochilus species by HPLC-ELSD

建立HPLC-ELSD法,测定三种植物基原金线莲中金线莲苷含量。采用phenomenex NH2色谱柱(250 mm×4.6 mm,5μm),以乙腈-水(92∶8,V/V)为流动相进行等度洗脱,流速为0.8 m L/min,柱温室温,ELSD雾化室温度为70℃,氮气流速为1.5 L/min。实验结果表明,金线莲苷进样量在76.02~2432.64μg/m L范围内线性良好(r=0.9991);精密度、稳定性、重复性实验的RSD均≤3%;平均加样回收率为97.55%,RSD为1.50%(n=6)。33批次金线莲的金线莲苷含量在6.37%~22.66%之间,平均含量为15.00%,存在较大的差异(RSD=26.29%)。该方法快速、简便,结果准确、可靠,适用于金线莲中金线莲苷的定量分析。

To establish a HPLC-ELSD method for determination of kinsenoside in Jin-Xian-Lian from three Anoectochilus species,the HPLC system consisting of phenomenex NHzcolumn（250 mm× 4.6 mm,5 μm）and an isocratic elution system of acetonitrile-water（92：8 ,V/V）as the mobile phase was adopted.The flow rate was set at 0.8 mL/min,the column temperature was room temperature, ELSD spray chamber temperature was 70 ℃, and the nitrogen flow rate was 1.5 L/rain. The results indicated that the liner range of kinsenoside was 76.02 - 2432.64 μg/mL （ r = 0.9991 ） ; RSD of precision, stability and reproducibility tests were no more than 3% ; average recovery rate was 97.55% （RSD = 1.50%, n = 6）.The contents of kinsenoside in Jin-Xian-Lian from three Anoectochilus species were in the range of 6.37% -22.66% ,and the average content was 15.00% （RSD = 26.29% ）. This method was quick, simple, accurate and reliable, and could be used to determine the content of kinsenoside in Jin-Xian-Lian.