水溶性壳聚糖对脂多糖诱导的巨噬细胞凋亡的影响

Effect of water soluble chitosan on the apoptosis of peritoneal macrophage induced by lipopolysaccharides

目的研究水溶性壳聚糖(water soluble chitosan,WSC)对脂多糖诱导的小鼠腹腔巨噬细胞凋亡的影响,并探讨其机制。方法无菌分离小鼠腹腔巨噬细胞,分为脂多糖刺激组、WSC与脂多糖共同作用组,并设磷酸盐缓冲液空白对照组,培养24 h后,流式细胞术检测细胞凋亡情况和活化胱天蛋白酶(caspase)-3的表达,Griess法测定一氧化氮释放量。结果各组细胞培养24 h后,与磷酸盐缓冲液空白对照组相比,脂多糖刺激组细胞的凋亡明显增加,一氧化氮的释放和caspase-3的活化明显增强;WSC与脂多糖共同作用组凋亡的巨噬细胞较脂多糖刺激组明显减少,一氧化氮的释放和caspase-3的活化也受到了明显的抑制。结论 WSC通过抑制一氧化氮的释放和caspase-3的活化阻止脂多糖诱导的巨噬细胞凋亡。

Objective To study the effect of water soluble chitosan（WSC） on the apoptosis of peritoneal macrophage induced by lipopolysaccharides（LPS）,and discuss the mechanism.Methods Peritoneal macrophages were divided to three groups：phosphate buffered saline（PBS） group,LPS group and LPS plus WSC group.At hour 24,apoptosis cell and active caspase-3 were detected by flow cytometry;nitric oxide（NO） was determined with Griess reagent.Results There were more apoptosis cells in the LPS group than the PBS group.The percentage of apoptosis cells was significantly decreased in the LPS plus WSC group than the LPS group.The expression of active caspase-3 and the secretion of NO were also inhibited by WSC after LPS intervention.Conclusion WSC inhibits apoptosis of peritoneal macrophage induced by LPS.