Notch信号通路在低声压次声促进骨髓间充质干细胞增殖中的作用

The role of Notch signaling pathway in the effect of infrasound on the multiplication of bone marrow mesenchymal stem cells

目的:探讨Notch信号通路在低声压次声对骨髓间充质干细胞(BMSCs)生物活性影响中的作用。方法:应用全骨髓培养法获取BMSCs,取第3代对数生长期的细胞,分为四组,分别为第1组(抑制剂+无次声组)、第2组(抑制剂+次声组)、第3组(无抑制剂+无次声组)、第4组(无抑制剂+次声组),按分组要求分别用γ-分泌酶抑制剂DAPT和低声压次声进行相应处理,利用MTT方法检测各组中骨髓间充质干细胞的增殖情况。结果:低声压次声作用后的24h、48h、72h和96h时,第3组(无抑制剂+无次声组)的OD值比第1组(抑制剂+无次声组)较大,第4组(无抑制剂+次声组)的OD值也大于第2组(抑制剂+次声组),比较均有显著性差异(P<0.05)。另外,低声压次声作用后24h、48h和72h时,第2组的OD值显著大于第1组的OD值(P<0.05);第4组的OD值也显著大于第3组的OD值(P<0.05)。随着作用时间的延长,四组细胞的OD值均呈递增趋势;在次声作用后24h、48h、72h、96h时,第4组的OD值均大于其他三组(4>3>2>1)。结论:低声压次声可促进BMSCs的增殖活性,Notch信号通路被抑制后会影响低声压次声对BMSCs的增殖作用,说明低声压次声促进骨髓间充质干细胞增殖的机制可能是通过激活Notch信号通路实现。

Objective： To investigate the role of Notch signaling pathway in the effect of infrasound on multiplication of bone marrow mesenchymal stem cells（BMSCs）.Method： The primary BMSCs were derived from Sprague-Dawley rats through whole bone marrow adherent cultivation. The cells of passage 3 were randomly divided into 4 groups with different treatments as follow：group 1（DAPT without infrasound）, group 2（DAPT with infrasound）, group 3（without any treatment） and group 4（infrasound without DAPT）. The proliferation of BMSCs in above 4 groups was measured by MTT assay.Result： After being treated with infrasound for 24 h, 48 h, 72 h and 96 h, the optical density（OD） value of group 3 is significantly higher than group 1, group 4 significantly higher than group 2（P〈0.05）. The OD value of group 2 treated with infrasound for 24 h, 48 h, 72 h is significantly higher than that in group 1, group 4higher than group 3（P〈0.05）. The OD value of four groups gradually increase and group 4 was the highest.Conclusion： Infrasound could promote the proliferation of BMSCs. What＇s more, such proliferative effects pro-moted by infrasound could be weakened when Notch signaling pathway was inhibited. It indicated that one of the mechanisms of infrasound promoting proliferation was activation of Notch signaling pathway.