HPLC法测定千里光总黄酮中金丝桃苷在大鼠体内的血药浓度及药动学研究

Plasma concentration of hyperoside in total Flavonoids of Senecionis scandens Herba in rats by HPLC and its pharmacokinetics study

〔目的〕建立高效液相色谱法(High Performance Liquid Chromatography,HPLC)测定千里光总黄酮中金丝桃苷在大鼠体内的血药浓度,并探讨其在大鼠体内药动学特征。〔方法〕SD大鼠给予千里光总黄酮灌胃,在不同时间点眼眶后静脉丛取血,预处理后测定血浆中金丝桃苷的浓度,用3P97软件处理数据。〔结果〕千里光总黄酮中金丝桃苷在大鼠体内的药动学符合二房室模型,金丝桃苷在体内较快地被吸收,给药后1 h即达到峰浓度,Cmax=(0.922±0.182)mg/L,其他主要药动学参数Ka=(2.754±0.382)/h、α=(1.421±0.275)/h、β=(0.238±0.083)/h、t1/2β=(11.084±5.962)h、AUC0-24=(9.271±2.357)mg·h·L-1)。〔结论〕高效液相色谱法测定血浆中金丝桃苷浓度准确、简便,适用于千里光总黄酮中金丝桃苷的药动学研究。

〔O bjective〕To establish a high performance liquid chromatography（ HPLC） method for measuring the concentration of hyperoside in total flavonoids of Senecionis scandens Herba in rats and the pharmacokinetics of which was also detected.〔Methods〕After oral administration of total flavonoids of Senecionis scandens Herba,blood sample were collected through venous plexus in rat eye at different time. After liquide extraction with acetonitrile, the concentration of hyperoside in plasma was measured. Then pharmacokinetics parameters were calculated with 3P97 program.〔R esults〕The result showed that hyperoside plasma concentration-time curves in rats coincided with two-compartment model, hyperoside was absorbed fast in rats. Cmaxof hyperoside in rat plasma was（ 0. 922 ± 0. 182） mg/L at one hour. The main pharmacokinetic parameters were composed of： Ka was（ 2. 754 ± 0. 382）/h, α was（ 1. 421 ± 0. 275）/h, β was（ 0. 238 ± 0. 083）/h, t1/2βwas（ 11. 084 ± 5. 962） h, AUC0-24was（ 9.271 ± 2. 357） mg· h· L^-1.〔C onclusion〕HPLC method is accurate and sensitive, and is suitable for the pharmacokinetic study of hyperoside in total flavonoids of Senecionis scandens Herba in rats.