基于液体发酵灵芝草乌相互作用的影响研究

Study on Interaction between Ganoderma lucidum and Aconiti kusnezoffii Based on Submerged Fermentation

[目的]研究在液体发酵过程中草乌与灵芝相互作用的影响。[方法]研究在灵芝液体发酵过程中初始p H、草乌粉及草乌提取物对灵芝菌体生长、胞内外多糖的影响,以及灵芝发酵对草乌粉及草乌提取物中酯型生物碱质量分数的影响。[结果]当初始p H为6时,灵芝菌体生物量及胞内外多糖产量均达最大值,分别为7.50、0.25、0.42 g/L;草乌粉添加量为20 g/L时,生物量和胞内多糖达最大,分别为10.60和0.54 g/L,分别为对照的1.96和2.57倍;草乌粉添加量为15 g/L时,胞外多糖产量达最大,为0.76 g/L,是对照的2.11倍;草乌提取物添加量为0.87 g/L时,生物量和胞外多糖产量达最大,分别为11.30和0.72 g/L,分别是对照的2.13和2.06倍,添加量为1.16 g/L时,胞内多糖产量最高,达0.57 g/L,是对照的2.38倍。[结论]灵芝均能降低发酵液中草乌粉、草乌提取物中的双酯型生物碱含量及增加发酵液中单酯型生物碱含量。

[ Objective ] The aim was to study interaction between Ganoderma lucidum and Aconiti kusnezoffii in liquid fermentation process. [Method] This paper explored the effect of initial pH,powder and total alkaloids extract of Aconiti kusnezoffii on the yield of mycelium and the content of intracellular polysaccharides（IPS） and extracellular polysaccharides（ EPS）, studied the effect of Ganoderma lucidum fermentation on the content of aconitum alkaloids in the submerged fermentation of Ganoderma lucidum. [ Result ] The yield of biomass and the content of IPS and EPS reached 7.50,0.25,0. 42 g/L respectively when initial pH was 6. The yield of biomass and the content of IPS reached 10.60,0. 54 g/L,and 1.96,2.57 times compared to the control respectively by the addition of 20 g/L of Aconiti kusnezoffii and the content of EPS was 0. 76 g/L,2.11 times compared to the control by the addition of 15 g/L. The yield of biomass and the content of EPS reached 1t. 30,0.72 g/L,2.13,2.06 times compared to the control respectively by the addition of 0. 87g/f. of the extract of Aconiti kusnezoffii and the content of IPS was O. 57g/L,2.38 times compared to the control by the addition of 1.16 g/L of extract of Aconiti kusnezoffii. [ Conclusion] The content of the diester-aconitum alka- loids was decreased and the content of mono-ester aconitum alkaloids was increased in Ganoderma lucidum submerged fermentation.