右美托咪定对梗阻性黄疸大鼠肝损伤的影响

Effect of dexmedetomidine on liver injury in rats with obstructive jaundice

目的评价右美托咪定对梗阻性黄疸大鼠肝损伤的影响。 方法健康雄性SD大鼠45只，体重250～300 g，8～9周龄，采用随机数字表法分为3组（n＝15）：假手术组（S组）、梗阻性黄疸组（OJ组）和右美托咪定组（D组）。OJ组及D组制备大鼠梗阻性黄疸模型，D组于造模后72 h时腹腔注射右美托咪定100 μg/kg。每组于给药后3、5和24 h时经心脏采集血样，测定血浆谷丙转氨酶（ALT）及C反应蛋白（CRP）的水平，每个时点采集血样后取肝组织，采用RT-PCR法检测肝组织Toll样受体4（TLR4） mRNA表达，采用ELISA法检测肝组织TLR4含量；HE染色，光学显微镜下观察肝组织病理学结果。 结果与S组比较，OJ组和D组给药后不同时点血浆ALT和CRP的水平升高，肝组织TLR4 mRNA表达上调，肝组织TLR4含量升高（P〈0.05）；与OJ组比较，D组给药后不同时点血浆ALT和CRP的水平降低，肝组织TLR4 mRNA表达下调，肝组织TLR4含量降低（P〈0.05）。D组肝组织损伤程度轻于OJ组，而重于S组。 结论右美托咪定可减轻梗阻性黄疸大鼠肝损伤。

Objective To evaluate the effect of dexmedetomidinc on liver injury in the rats with ob- structive jaundice. Methods Forty-five healthy male Sprague Dawley rats, weighing 250-300 g, aged 8- 9 weeks, were divided into 3 groups （n = 15 each） using a random number table： sham operation group （S group） , obstructive jaundice group （OJ group） and dexmedetomidine group （D group）. Obstructive jaun- t｜ice was induced in rats by division and double ligation of the common bile duct in OJ and D groups. Dexmedetomidine 100 ixg/kg was injected intraperitoneally at 72 h after establishment of the model in group D. At 3, 5 and 24 h after administration, blood samples were collected from hearts for determination of the plasma alanine aminotransferase （ALT） and C-reactive protein （CRP） levels. After blood sampling at each time point, the specimens from the external right lobe of the liver were obtained for detection of the expres- sion of Toll-like receptor 4 （TLR4） mRNA （by real-time polymerase chain reaction） and TLR4 content （by enzyme-linked immunosorbcnt assay） in liver tissues and for pathological examination of liver tissues （with light microscope）. Results Compared with group S, the plasma ALT and CRP levels were significantly increased at each time point after administration, and the expression of TLR4 mRNA in liver tissues was significantly up-regulated, and TLR4 content in liver tissues was significantly increased in OJ and D groups （ P〈0.05）. Compared with group OJ, the plasma ALT and CRP levels were significantly decreased at each time point after administration, and the expression of TLR4 mRNA in liver tissues was significantly down- regulated, and TLR4 content in liver tissues was significantly decreased in group D （P〈0.05）. The degree of damage to liver tissues was significantly attenuated in group D compared with group O J, and was aggrava- ted in group D compared with group S. Conclusion Dexmedetomidine can reduce liver injury in the rats with obstructive jaundice.