侧脑室注射腺苷A1受体激动剂对硝酸甘油偏头痛模型大鼠的影响

Analgesic effect of adenosine A1 receptor agonist into lateral ventricles in rat models of migraine

目的通过对硝酸甘油偏头痛模型大鼠侧脑室注射腺苷A1受体激动剂(R-phenylisopropyl-adenosine,R-PIA),探讨腺苷在偏头痛中的作用及机制。方法观察各组大鼠不同时间段内挠头、爬笼次数及心率变化,采用Elisa、免疫组织化学法、Western blot技术检测血液、三叉神经节(trigeminal ganglion,TG)、三叉神经脊束核尾核(spinal trigeminal nucleus caudalis,TNC)处降钙素基因相关肽(calcitonin gene-related peptide,CGRP)的表达。结果(1)与模型组相比,治疗组大鼠挠头、爬笼次数均减少并呈剂量依赖性(P<0.05),且大鼠心率没有明显变化;(2)治疗组大鼠血液、TG、TNC部位CGRP的表达较模型组均降低(P<0.05)。结论激活的腺苷A1受体能够通过抑制神经源性炎症反应及痛觉传导,从而抑制三叉神经血管系统的激活及痛觉敏化,对偏头痛产生镇痛作用。

Objective To investigate the mechanism of the adenosine in the treatment of migraine ,the adenosine A1 receptor agonist was injected into the lateral ventricles of rats of migraine .Methods Observed the numbers of the rats scratching their heads , climbing cage times and heart rate changes , and the ELISA , immunohistochemistry , Western blot techniques were used to detect the expression of the calcitonin gene -related peptide （ CGRP ） .Results Compaired with model group , R-PIA produced a dose-dependent manner of the behavioral manifestations and the expression of CGRP . Conclusion Adenosine A1 receptor agonist can inhibit the activation of trigeminal vascular system by inhibiting the neuro -genic inflammation reaction and trigeminovascular nociceptive transmission ,which can produce analgesic effect on migraine .