兔眼蓝莓离体叶片再生组织细胞学观察

Histological and Cellular Observations of Regeneration from Leaf Explants of Rabbiteye Blueberry(Vaccinium ashei Reade) in vitro

离体叶片再生是兔眼蓝莓离体快繁和遗传转化的重要途径。为了探明兔眼蓝莓离体叶片再生途径,以兔眼蓝莓杰兔品种的试管苗叶片为外植体,对离体叶片再生途径进行细胞学观察。结果表明,离体叶片不定芽在30 d内基本完成其发生、发育和形成的全过程。离体叶片以直接再生途径发生不定芽,且属于多起源,其分生组织起源于离体叶片切口附近与维管束相邻的上表皮细胞、维管组织薄壁细胞及周围薄壁细胞,通过细胞分裂和分化形成分生细胞团,直接形成芽,再发育成苗。此外,兔眼蓝莓离体叶片不定芽的形成具有特定的时空特性,多个不定芽先后在离体叶片上形成单芽或丛生芽。

The leaf regeneration in vitro is an important way of rapid propagation and genetic transformation of rabbiteye blueberry.The regeneration pathway for leaf explants of rabbiteye blueberry were firstly clarified by tissue culture and paraffin section techniques,in which the tube plantlet leaves of rabbiteye blueberry＂Premier＂were used as explants.The results showed that the adventitious buds on the leaf explants could basically be formed within 30 d in vitro culture,going through the processes of occurrence,development and formation.The adventitious buds were regenerated from different tissues of leaf explants of rabbiteye blueberry by direct regeneration way.The meristems originated from the epidermal cells,the parenchyma ones of vascular tissues,and the surrounding ones,which formed meristematic cells by cellular division and differentiation and then formed buds and shoots directly.What＇s more,the adventitious buds were successively produced with single buds or multiple shoot clumps on leaf explants,which indicates that the formation of adventitious buds had some particular space-time characteristics.