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对BHK-21悬浮细胞流加培养的研究

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摘要 目的 分析和探讨培养基中不同的营养成分对BHK-21悬浮细胞流加培养的影响.方法 首先进行BHK-21悬浮细胞的复苏与摇床传代培养,其次设置3个实验组,每个实验组含有不同的营养成分,并以批次培养对照.结果 3个实验组中含有氨基酸的一组对细胞的增长有促进作用,含有无血清培养基的一组既能够促进细胞的增长又能维持细胞的活力.结论 氨基酸和无血清成分均能促进细胞的生长,后者更具有维持细胞活力的作用,适合培养BHK-21悬浮细胞.
作者 邹鲁
出处 《农业科技与信息》 2016年第33期137-138,共2页 Agricultural Science-Technology and Information
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